Kim K I, Kim Y S, Kim H K, Chae Y S, Yoem B W, Kim I
Department of Pathology, College of Medicine, Korea University, Seoul.
Pathol Res Pract. 1999;195(6):407-12. doi: 10.1016/S0344-0338(99)80014-4.
Epstein-Barr virus (EBV) is known in association with lymphoid and epithelial lesion. Because the salivary gland is an organ close to the oropharynx, it has a higher incidence of EBV infection and is a possible route of EBV infection. Formalin-fixed, paraffin embedded tissue sections of 87 cases of salivary gland diseases were used for the study of EBV with PCR, in situ PCR for EBNA-1 (EBV nuclear antigen-1), and immunohistochemistry for LMP-1 (latent membrane protein-1). EBV was detected in 12 cases (13.8%): 7 of nonspecific chronic sialadenitis (21.2%), 4 of Warthin's tumors (30.8%), and one lymphoepithelial carcinoma. EBNA-1 was negative in all the other lesions. EBV DNA was detected in the nucleus of epithelial cells and the surrounding lymphocytes. LMP-1 positivity was found in the cytoplasm of epithelial cells. The results of the present study showed that EBV is implicated in some of the inflammatory and neoplastic lesions of the salivary gland in which the lymphocytes are abundant. However, the pathogenesis and mechanism of immortalization and tumorigenesis of the epithelial cells in the salivary glands remain to be determined.
爱泼斯坦-巴尔病毒（EBV）与淋巴样和上皮性病变相关。由于唾液腺是靠近口咽的器官，其EBV感染发生率较高，是EBV感染的一条可能途径。本研究采用聚合酶链反应（PCR）、EBNA-1（EBV核抗原-1）原位PCR及LMP-1（潜伏膜蛋白-1）免疫组化方法，对87例唾液腺疾病的福尔马林固定、石蜡包埋组织切片进行EBV研究。12例（13.8%）检测到EBV：非特异性慢性涎腺炎7例（21.2%），沃辛瘤4例（30.8%），淋巴上皮癌1例。所有其他病变中EBNA-1均为阴性。上皮细胞核及周围淋巴细胞中检测到EBV DNA。上皮细胞胞质中发现LMP-1阳性。本研究结果表明，EBV与唾液腺中一些淋巴细胞丰富的炎症性和肿瘤性病变有关。然而，唾液腺上皮细胞永生化和肿瘤发生的发病机制及机制仍有待确定。
