Wu Lan-yan, Cheng Jun, Lu Yong, Zhou Zhi-yu, Saku T
Department of Pathology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2004 Jul;39(4):291-3.
To examine the role of Epstein-Barr virus (EBV) infection in benign lymphoepithelial lesions with malignant transformation of salivary glands.
2 cases benign lymphoepithelial lesions with focal malignant transformation (BLEL-FMT), 14 cases of malignant lymphoepithelial lesion (MLEL) and 4 cases of benign lymphoepithelial lesion (BLEL) of salivary glands, 4 cases of nonspecific chronic sialadenitis were examined. Sections were cut from the paraffin blocks and performed in situ hybridization (ISH) for EBV encoded small RNA-1 (EBER-1) using 30-base synthesized oligonucleotide probe and polymerase chain reaction (PCR) for BamHI-W regein of EBV DNA (123 bp).
In ISH, strong signals for EBER-1 were obtained in most of epithelial cell nuclei in examined specimens of BLEL-FMT, no matter in benign or malignant areas and in all specimens of MLEL (14/14), PCR demonstrated a DNA fragment of 123 kbp in 2 cases of BLEL-FMT and 12 cases of MLEL (12/14). In contrast, there were no significant ISH/PCR signals in examined BLEL and nonspecific chronic sialadenitis.
A few cases of MLEL may arise from BLEL and EBV may plays an important role in the pathogenesis of BLEL-FMT of salivary glands.
探讨爱泼斯坦-巴尔病毒（EBV）感染在涎腺良性淋巴上皮病变恶变中的作用。
对2例伴有局灶性恶变的良性淋巴上皮病变（BLEL-FMT）、14例恶性淋巴上皮病变（MLEL）、4例涎腺良性淋巴上皮病变（BLEL）以及4例非特异性慢性涎腺炎进行研究。从石蜡块中切片，使用30个碱基的合成寡核苷酸探针进行EBV编码小RNA-1（EBER-1）的原位杂交（ISH），并对EBV DNA的BamHI-W区域（123 bp）进行聚合酶链反应（PCR）。
在ISH中，BLEL-FMT的大多数上皮细胞核中均获得了EBER-1的强信号，无论在良性还是恶性区域，且在所有MLEL标本中（14/14）均有信号；PCR显示2例BLEL-FMT和12例MLEL（12/14）中有123 kbp的DNA片段。相比之下，在检测的BLEL和非特异性慢性涎腺炎中未发现明显的ISH/PCR信号。
少数MLEL可能起源于BLEL，EBV可能在涎腺BLEL-FMT的发病机制中起重要作用。
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