在病毒重建过程中从BAC克隆的疱疹病毒基因组中系统切除载体序列。
Systematic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution.
作者信息
Wagner M, Jonjic S, Koszinowski U H, Messerle M
机构信息
Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität München, D-81377 Munich, Germany.
出版信息
J Virol. 1999 Aug;73(8):7056-60. doi: 10.1128/JVI.73.8.7056-7060.1999.
Abstract
Recently the mouse cytomegalovirus (MCMV) genome was cloned as an infectious bacterial artificial chromosome (BAC) (M. Messerle, I. Crnkovic, W. Hammerschmidt, H. Ziegler, and U. H. Koszinowski, Proc. Natl. Acad. Sci. USA 94:14759-14763, 1997). The virus obtained from this construct is attenuated in vivo due to deletion of viral sequences and insertion of the BAC vector. We reconstituted the full-length MCMV genome and flanked the BAC vector with identical viral sequences. This new construct represents a versatile basis for construction of MCMV mutants since virus generated from the construct loses the bacterial sequences and acquires wild-type properties.
摘要
最近，小鼠巨细胞病毒（MCMV）基因组被克隆为一种感染性细菌人工染色体（BAC）（M. 梅塞尔勒、I. 克尔恩科维奇、W. 哈默施密特、H. 齐格勒和U. H. 科斯齐诺夫斯基，《美国国家科学院院刊》94:14759 - 14763，1997年）。由于病毒序列的缺失和BAC载体的插入，从该构建体获得的病毒在体内减毒。我们重构了全长MCMV基因组，并在BAC载体两侧加上相同的病毒序列。这种新的构建体是构建MCMV突变体的通用基础，因为从该构建体产生的病毒会丢失细菌序列并获得野生型特性。
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