Kemble G, Duke G, Winter R, Spaete R
Aviron, Mountain View, California 94043, USA.
J Virol. 1996 Mar;70(3):2044-8. doi: 10.1128/JVI.70.3.2044-2048.1996.
We have constructed defined human cytomegalovirus (CMV) mutants by cotransfecting overlapping cosmid clones spanning the 230-kbp genome. Using this strategy, we have introduced a 13-kbp region of DNA from a virulent strain of CMV into a defined position within the avirulent CMV(Towne) genome. Although more than 80% of the genome of these recombinant viruses was derived from Towne DNA, their plaque morphology more closely resembled that of Toledo. To date, CMV is the largest virus and requires the greatest number of cosmids to be regenerated via overlapping cosmid cotransfection.
我们通过共转染跨越230千碱基对基因组的重叠黏粒克隆构建了明确的人巨细胞病毒（CMV）突变体。利用这一策略，我们已将来自CMV强毒株的一段13千碱基对的DNA区域引入无毒CMV（汤氏株）基因组内的一个特定位置。尽管这些重组病毒基因组的80%以上源自汤氏株DNA，但其蚀斑形态更类似于托莱多株。迄今为止，CMV是最大的病毒，通过重叠黏粒共转染再生所需的黏粒数量最多。
