通过共转染重叠黏粒构建的人巨细胞病毒基因组的大规模特定改变。
Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids.
作者信息
Kemble G, Duke G, Winter R, Spaete R
机构信息
Aviron, Mountain View, California 94043, USA.
出版信息
J Virol. 1996 Mar;70(3):2044-8. doi: 10.1128/JVI.70.3.2044-2048.1996.
Abstract
We have constructed defined human cytomegalovirus (CMV) mutants by cotransfecting overlapping cosmid clones spanning the 230-kbp genome. Using this strategy, we have introduced a 13-kbp region of DNA from a virulent strain of CMV into a defined position within the avirulent CMV(Towne) genome. Although more than 80% of the genome of these recombinant viruses was derived from Towne DNA, their plaque morphology more closely resembled that of Toledo. To date, CMV is the largest virus and requires the greatest number of cosmids to be regenerated via overlapping cosmid cotransfection.
摘要
我们通过共转染跨越230千碱基对基因组的重叠黏粒克隆构建了明确的人巨细胞病毒（CMV）突变体。利用这一策略，我们已将来自CMV强毒株的一段13千碱基对的DNA区域引入无毒CMV（汤氏株）基因组内的一个特定位置。尽管这些重组病毒基因组的80%以上源自汤氏株DNA，但其蚀斑形态更类似于托莱多株。迄今为止，CMV是最大的病毒，通过重叠黏粒共转染再生所需的黏粒数量最多。
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本文引用的文献
1
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J Virol. 1996 Jan;70(1):78-83. doi: 10.1128/JVI.70.1.78-83.1996.
2
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Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7376-80. doi: 10.1073/pnas.90.15.7376.
3
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4
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Virology. 1993 Nov;197(1):116-24. doi: 10.1006/viro.1993.1572.
5
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J Virol. 1994 Aug;68(8):4837-46. doi: 10.1128/JVI.68.8.4837-4846.1994.
6
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J Virol. 1995 Jan;69(1):589-96. doi: 10.1128/JVI.69.1.589-596.1995.
7
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Ann Intern Med. 1984 Oct;101(4):478-83. doi: 10.7326/0003-4819-101-4-478.
8
A cytomegalovirus protein with properties of herpes simplex virus ICP8: partial purification of the polypeptide and map position of the gene.一种具有单纯疱疹病毒ICP8特性的巨细胞病毒蛋白：该多肽的部分纯化及基因的图谱定位
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9
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10
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