氟烷对大鼠皮层切片中钾离子刺激的乙酰胆碱释放的抑制作用。
Inhibition by halothane of potassium-stimulated acetylcholine release from rat cortical slices.
作者信息
Griffiths R, Greiff J M, Haycock J, Elton C D, Rowbotham D J, Norman R I
机构信息
Department of Anaesthesia, University of Leicester, Leicester Royal Infirmary.
出版信息
Br J Pharmacol. 1995 Oct;116(4):2310-4. doi: 10.1111/j.1476-5381.1995.tb15070.x.
Abstract
- Cholinergic neurones in the basal forebrain are linked to cortical activation and arousal. 2. The present study was designed to examine the hypothesis that clinically relevant doses of halothane (0.1 to 5%) would significantly reduce depolarization-evoked acetylcholine (ACh) release from rat cortical slices. 3. ACh release was measured from rat cortical slices by a chemiluminescent technique. 4. Depolarization-evoked ACh release was inhibited significantly by halothane with an IC50 of 0.38%. This value equates to 0.3 MAC (the minimum alveolar concentration at which no movement occurs to a standard surgical stimulus in 50% of subjects) for the rat. 5. The potent effect of halothane on ACh release suggests that this mechanism may be a target for the action of volatile anaesthetic agents. This in vitro effect on ACh release is consistent with effects of halothane reported in vivo.
摘要
- 基底前脑的胆碱能神经元与皮层激活和觉醒相关。2. 本研究旨在检验以下假设：临床相关剂量的氟烷（0.1%至5%）会显著减少大鼠皮层切片中去极化诱发的乙酰胆碱（ACh）释放。3. 通过化学发光技术测量大鼠皮层切片中的ACh释放。4. 氟烷显著抑制去极化诱发的ACh释放，IC50为0.38%。该值相当于大鼠的0.3MAC（50%的受试者对标准外科刺激无运动反应时的最低肺泡浓度）。5. 氟烷对ACh释放的强效作用表明该机制可能是挥发性麻醉剂作用的靶点。这种对ACh释放的体外作用与体内报道的氟烷作用一致。
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