Tang K F, Chan S H, Loh K S, Chong S M, Wang D, Yeoh K H, Hu H
Department of Microbiology, Faculty of Medicine, The National University of Singapore, Singapore.
Cancer Lett. 1999 Jun 1;140(1-2):93-8. doi: 10.1016/s0304-3835(99)00056-7.
Undifferentiated nasopharyngeal carcinomas (UNPC) are characterised by an association with Epstein-Barr virus and an abundant lymphoid stroma. We studied the functional status of the infiltrating T cells in ten UNPC biopsies using an immunohistochemical approach. Twelve non-NPC biopsies were included as controls. Tumour cells of UNPC were positive for HLA class I (10/10) and II (8/10), LMP1 (3/10), and CD86 (6/10). Tumour infiltrating T cells (TILs) were detected with antibodies directed at CD3, CD4, and CD8, and shown to be comparable to that in the control biopsies. Although expression of CD28 was shown to be decreased in TILs, expression of CD25 and IFN-gamma at a relatively high percentage could be consistently detected in the UNPC biopsies. These data suggest that TILs in UNPC are in an activated status, and this T cell response is possibly directed at the tumour cells.
未分化鼻咽癌（UNPC）的特征是与爱泼斯坦-巴尔病毒相关以及有丰富的淋巴样基质。我们采用免疫组织化学方法研究了10例UNPC活检组织中浸润性T细胞的功能状态。纳入12例非NPC活检组织作为对照。UNPC的肿瘤细胞HLA I类（10/10）和II类（8/10）、LMP1（3/10）以及CD86（6/10）呈阳性。用针对CD3、CD4和CD8的抗体检测肿瘤浸润性T细胞（TILs），结果显示其与对照活检组织中的情况相当。尽管TILs中CD28的表达降低，但在UNPC活检组织中可始终检测到相对较高比例的CD25和IFN-γ表达。这些数据表明，UNPC中的TILs处于激活状态，这种T细胞反应可能针对肿瘤细胞。
