Lai F M, Cheng P N, Tsao S Y, Lai K N
Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin.
Virchows Arch A Pathol Anat Histopathol. 1990;417(4):347-52. doi: 10.1007/BF01605787.
The immunohistological characteristics of infiltrating lymphoid cells and the expression of human leucocyte antigens class I and II (HLA-ABC and HLA-DR, respectively) were studied in 50 pre-treatment nasopharyngeal carcinomas. The majority of lymphoid cells were activated lymphocytes expressing thymocyte OKT10 marker. CD4+ cells (T-helper/inducer) out-numbered CD8+ cells (T-suppressor/cytotoxic) by at least two- to four-fold. CD22+ cells (pan-B lymphocytes) were scanty in the peri-tumoral areas and were absent in 29 out of 50 biopsies. A moderate number of cells expressing CD15 (monocytes/macrophages) were also detected. CD16+ cells (natural killer cells) were found to be sparse or absent. Expression of HLA class I and II antigens on the tumor cells in 35 biopsies was variable. HLA-ABC staining was intense in 6, reduced in 13 and partially lost in 16, whereas staining of HLA DR was intense in 7, reduced in 11 and partially lost in 17. Full expression of both antigens was demonstrable in only 2 biopsy samples. The expression of HLA antigens in the tumour had no relationship to the type or degree of lymphocytic infiltration or staging of the tumour.
对50例治疗前鼻咽癌患者的浸润性淋巴细胞免疫组织学特征以及人类白细胞抗原I类和II类（分别为HLA - ABC和HLA - DR）的表达情况进行了研究。大多数淋巴细胞为表达胸腺细胞OKT10标志物的活化淋巴细胞。CD4 + 细胞（辅助性/诱导性T细胞）数量至少是CD8 + 细胞（抑制性/细胞毒性T细胞）的两到四倍。CD22 + 细胞（全B淋巴细胞）在肿瘤周边区域稀少，50份活检标本中有29份未检测到。还检测到中等数量表达CD15的细胞（单核细胞/巨噬细胞）。发现CD16 + 细胞（自然杀伤细胞）稀少或未检测到。35份活检标本中肿瘤细胞上HLA I类和II类抗原的表达情况各不相同。HLA - ABC染色强阳性的有6份，减弱的有13份，部分缺失的有16份；而HLA - DR染色强阳性的有7份，减弱的有11份，部分缺失的有17份。仅在2份活检样本中可证实两种抗原均完全表达。肿瘤中HLA抗原的表达与淋巴细胞浸润的类型或程度以及肿瘤分期无关。
