Belknap E B, Walters L M, Kelling C, Ayers V K, Norris J, McMillen J, Hayhow C, Cochran M, Reddy D N, Wright J, Collins J K
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
Vaccine. 1999 May 4;17(18):2297-305. doi: 10.1016/s0264-410x(98)00466-6.
The efficacy and safety of a gene-deleted bovine herpesvirus-1 (BHV-1) vaccine was determined in a bovine herpesvirus challenge trial in calves. Three different doses of the vaccine were administered intramuscularly at 10(5), 10(6) and 10(7) PFU/ml and compared to a commercial vaccine and non vaccinated control calves. Challenge was performed by intranasal aerosolization with the Cooper strain of BHV-1 (3 x 10(4) PFU/ml). The non-vaccinated calves shed significantly (P < 0.05) more virus than all other groups on days 4, 8 and 10 post challenge. By day 14 post challenge, antibody titers for BHV-1 of calves vaccinated with 10(7) PFU/ml were significantly (P < 0.05) higher than the commercial or non-vaccinated calves. Clinical scores of non-vaccinated calves were significantly (P < 0.05) higher than all other groups on days 4-14 post challenge. With both radioimmunoprecipitation and competitive enzyme-linked immunosorbent assays (C-ELISA), calves in the gene-deleted vaccine groups mounted comparable specific responses against gB, gC and gD post vaccination as calves in the commercial vaccine group, but in a dose dependent manner. These data suggest that the gene-deleted BHV-1 vaccine tested may be used as an effective vaccine in controlling BHV-1 infections.
在一项针对犊牛的牛疱疹病毒攻毒试验中，测定了一种基因缺失的牛疱疹病毒1型（BHV-1）疫苗的有效性和安全性。以10(5)、10(6)和10(7) PFU/ml的三种不同剂量肌肉注射该疫苗，并与一种商业疫苗和未接种疫苗的对照犊牛进行比较。用BHV-1的库珀毒株（3×10(4) PFU/ml）通过鼻内雾化进行攻毒。在攻毒后第4、8和10天，未接种疫苗的犊牛排出的病毒明显（P<0.05）多于所有其他组。到攻毒后第14天，接种10(7) PFU/ml疫苗的犊牛的BHV-1抗体滴度明显（P<0.05）高于商业疫苗组或未接种疫苗的犊牛。在攻毒后第4至14天，未接种疫苗的犊牛的临床评分明显（P<0.05）高于所有其他组。通过放射免疫沉淀和竞争酶联免疫吸附测定（C-ELISA），基因缺失疫苗组的犊牛在接种疫苗后对gB、gC和gD产生了与商业疫苗组犊牛相当的特异性反应，但呈剂量依赖性。这些数据表明，所测试的基因缺失BHV-1疫苗可作为控制BHV-1感染的有效疫苗。
