Larochelle R, Antaya M, Morin M, Magar R
Laboratoire d'Hygiène Vétérinaire et Alimentaire, Agence Canadienne d'Inspection des Aliments, Saint-Hyacinthe, Québec, Canada.
J Virol Methods. 1999 Jun;80(1):69-75. doi: 10.1016/s0166-0934(99)00032-4.
A multiplex PCR assay was developed to detect and differentiate between the porcine circovirus (PCV) infecting persistently the PK 15 cell line (PCV type I) and the PCV associated with postweaning multisystemic wasting syndrome (PMWS) (PCV type II). DNA products with unique sizes characteristic of each type of PCV were obtained. Sequencing of these products demonstrated that the nucleotide sequences were type-specific. Tissue samples from a total of 42 field cases from Québec were studied, among which 41 were collected in 1997-1998 and one which had been previously collected in 1994. These 42 cases found previously to be PCV-positive by PCR were tested in the present study by a multiplex PCR assay to determine the type of PCV in each case. From these 42 field cases, 40 cases were PCV type II-positive, one case was PCV type I-positive and one case was positive for both PCV types I and II. PCV type II was identified in typical PMWS field cases, but also in field cases submitted for various clinical histories, some of which were not suggestive of PMWS. In the field case where PCV type I was detected, there was no clinical evidence nor histological lesions suggestive of PMWS. The demonstration of PCV type II in a total of 41/42 field cases in the present study suggests that PCV type II may be the main type of PCV circulating in pigs. Furthermore the detection of PCV type II in a field case dating back to 1994 indicates that this PCV type was circulating in pigs in Québec several years before the report of clinical PMWS in this province.
开发了一种多重聚合酶链反应（PCR）检测方法，用于检测和区分持续感染PK-15细胞系的猪圆环病毒（PCV，I型）和与断奶后多系统消耗综合征（PMWS）相关的PCV（II型）。获得了具有每种PCV类型独特大小特征的DNA产物。对这些产物进行测序表明，核苷酸序列具有类型特异性。研究了来自魁北克的总共42例田间病例的组织样本，其中41例于1997 - 1998年采集，1例于1994年采集。本研究通过多重PCR检测方法对之前经PCR检测为PCV阳性的这42例病例进行检测，以确定每例病例中PCV的类型。在这42例田间病例中，40例为II型PCV阳性，1例为I型PCV阳性，1例I型和II型PCV均为阳性。II型PCV在典型的PMWS田间病例中被鉴定出来，但在因各种临床病史送检的田间病例中也有发现，其中一些病例并无PMWS的迹象。在检测到I型PCV的田间病例中，没有临床证据或组织学病变提示PMWS。本研究中42例田间病例中有41例检测到II型PCV，这表明II型PCV可能是猪群中循环的主要PCV类型。此外，在1994年的一例田间病例中检测到II型PCV，这表明该PCV类型在魁北克猪群中传播早在该省临床PMWS报告出现的数年前就已存在。
