Choi Jiwon, Stevenson Gregory W, Kiupel Matti, Harrach Balázs, Anothayanontha Lavun, Kanitz Charles L, Mittal Suresh K
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907 USA.
Can J Vet Res. 2002 Oct;66(4):217-24.
The entire genomes of 7 isolates of porcine circovirus (PCV) from pigs with congenital tremors (CT), type A2, or postweaning multisystemic wasting syndrome (PMWS) were cloned and sequenced. One isolate (CT-PCV-P7) originated from the late 1960s from a neonatal pig with CT, type A2. Two recent PCV isolates (CT-PCV-P5, CT-PCV-P6) were from 2 affected neonatal pigs, from different farms, with unrelated outbreaks of CT; type A2. Four isolates (PMWS-PCV-P1, PMWS-PCV-P2, PMWS-PCV-P3, PMWS-PCV-P4) originated from pigs with PMWS from 4 different farms. A comparative analysis of these PMWS and PCV isolates demonstrated 99% sequence identity with each other, and over 96% sequence identity with previously sequenced PCV2 isolates. The CT-PCV-P5 and CT-PCV-P6 isolates, however, shared 99% of the same identity with each other, and interestingly also with PMWS PCV isolates. There were no consistent genomic differences between PMWS and recent CT isolates. The CT-PCV-P7 showed 98% identity similarity to PK-15-derived PCV1 and demonstrated only 72% identity similarity to either CT-PCV-P5 or CT-PCV-P6. Phylogenetic analysis confirmed that the old isolate (CT-PCV-P7), and the new isolates (CT-PCV-P5, CT-PCV-P6, PMWS-PCV-P1, PMWS-PCV-P2, PMWS-PCV-P3, PMWS-PCV-P4) were correctly classified as PCV1 and PCV2, respectively.
对7株来自患有先天性震颤（CT）、A2型或断奶后多系统消耗综合征（PMWS）的猪的猪圆环病毒（PCV）的全基因组进行了克隆和测序。其中一株分离株（CT - PCV - P7）于20世纪60年代末来自一头患有A2型CT的新生仔猪。另外两株近期的PCV分离株（CT - PCV - P5、CT - PCV - P6）分别来自两个不同农场的2头受影响的新生仔猪，它们发生了无关的CT疫情；均为A2型。四株分离株（PMWS - PCV - P1、PMWS - PCV - P2、PMWS - PCV - P3、PMWS - PCV - P4）分别来自4个不同农场患有PMWS的猪。对这些PMWS和PCV分离株的比较分析表明，它们彼此之间的序列同一性为99%，与先前测序的PCV2分离株的序列同一性超过96%。然而，CT - PCV - P5和CT - PCV - P6分离株彼此之间的同一性为99%，有趣的是，它们与PMWS PCV分离株的同一性也为99%。PMWS和近期CT分离株之间没有一致的基因组差异。CT - PCV - P7与源自PK - 15的PCV1的同一性相似性为98%，与CT - PCV - P5或CT - PCV - P6的同一性相似性仅为72%。系统发育分析证实，旧的分离株（CT - PCV - P7）和新的分离株（CT - PCV - P5、CT - PCV - P6、PMWS - PCV - P1、PMWS - PCV - P2、PMWS - PCV - P3、PMWS - PCV - P4）分别被正确分类为PCV1和PCV2。
