烟草转化酶抑制剂同源物的异位表达可防止马铃薯块茎冷诱导糖化。
Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers.
作者信息
Greiner S, Rausch T, Sonnewald U, Herbers K
机构信息
Botanisches Institut, INF 360, Heidelberg, Germany.
出版信息
Nat Biotechnol. 1999 Jul;17(7):708-11. doi: 10.1038/10924.
We have transformed potato with Nt-inhh cDNA, encoding a putative vacuolar homolog of a tobacco cell wall invertase inhibitor, under the control of the CaMV 35S promoter. In transgenic tubers, cold-induced hexose accumulation was reduced by up to 75%, without any effect on potato tuber yield. Processing quality of tubers was greatly improved without changing starch quantity or quality, an important prerequisite for the biotechnological use of Nt-inhh for potato transformation.
我们已在花椰菜花叶病毒35S启动子的控制下，用编码烟草细胞壁转化酶抑制剂假定液泡同源物的Nt-inhh cDNA转化了马铃薯。在转基因块茎中，冷诱导的己糖积累减少了多达75%，而对马铃薯块茎产量没有任何影响。块茎的加工品质得到了极大改善，同时淀粉的数量和质量没有改变，这是将Nt-inhh用于马铃薯转化的生物技术应用的一个重要前提。
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