Chalmers J J, Haam S, Zhao Y, McCloskey K, Moore L, Zborowski M, Williams P S
Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA.
Biotechnol Bioeng. 1999 Sep 5;64(5):509-18. doi: 10.1002/(sici)1097-0290(19990905)64:5<509::aid-bit1>3.0.co;2-z.
An experimental technique is discussed in which the size distribution of a population of cells is determined by calculating each cell's settling velocity. The settling velocity is determined from microscopically obtained images which were recorded on SVHS tape. These images are then computer imaged and processed, and the cell's location and velocity are determined using a computer algorithm referred to as cell tracking velocimetry (CTV). Experimental data is presented comparing the distribution of human lymphocytes and a human breast cancer cell line, MCF-7, determined using a Coulter counter and the CTV approach.
本文讨论了一种实验技术，即通过计算每个细胞的沉降速度来确定细胞群体的大小分布。沉降速度由记录在超级 VHS 录像带上的显微镜图像确定。然后对这些图像进行计算机成像和处理，并使用一种称为细胞跟踪测速法（CTV）的计算机算法来确定细胞的位置和速度。文中给出了实验数据，比较了使用库尔特计数器和 CTV 方法测定的人类淋巴细胞和人乳腺癌细胞系 MCF-7 的分布情况。
