Chalmers J J, Haam S, Zhao Y, McCloskey K, Moore L, Zborowski M, Williams P S
Department of Chemical Engineering, The Ohio State University, 140 WEST 19th Avenue, Columbus, Ohio 43210, USA.
Biotechnol Bioeng. 1999 Sep 5;64(5):519-26.
An experimental technique is discussed in which the magnetic susceptibility of immunomagnetically labeled cells can be determined on a cell-by-cell basis. This technique is based on determining the magnetically induced velocity that an immunomagnetically labeled cell has in a well-defined magnetic energy gradient. This velocity is determined through the use of video recordings of microscopic images of cells moving in the magnetic energy gradient. These video images are then computer digitized and processed using a computer algorithm, cell tracking velocimetry, which allows larger numbers (>10(3)) of cells to be analyzed.
本文讨论了一种实验技术，通过该技术可以逐个细胞地测定免疫磁标记细胞的磁化率。该技术基于测定免疫磁标记细胞在明确的磁能梯度中所具有的磁诱导速度。此速度是通过对细胞在磁能梯度中移动的微观图像进行视频记录来确定的。然后，这些视频图像通过计算机算法——细胞跟踪测速法进行数字化和处理，该算法能够分析大量（>10³）的细胞。
