Roberts D, Smith D J
Department of Biology, Faculty of University Transfer Sciences, Selkirk College, Castlegar, British Columbia, Canada.
J Sports Sci. 1999 Jun;17(6):485-93. doi: 10.1080/026404199365795.
The aim of this study was to determine if the hypoxaemic stimulus generated by intense exercise results in the physiological response of increased erythropoietin production. Twenty athletes exercised for 3 min at 109 +/- 2.8% (mean +/- s) maximal oxygen consumption. Estimated oxyhaemoglobin saturation was measured by reflective probe pulse oximetry (Nellcor N200) and was validated against arterial oxyhaemoglobin saturation by CO-oximetry in eight athletes. Serum erythropoietin concentrations-as measured using the INCSTAR Epo-Trac radioimmunoassay-increased significantly by 28 +/- 9% at 24 h post-exercise in 11 participants, who also had an arterial oxyhaemoglobin saturation < or = 91% (P < 0.05). Decreased ferritin levels and increased reticulocyte counts were observed at 96 h post-exercise. However, no significant changes in erythropoietin levels were observed in nine non-desaturating athletes and eight non-exercise controls. Good agreement was shown between arterial oxyhaemoglobin saturation and percent estimated oxyhaemoglobin saturation (limits of agreement = -3.9 to 3.7%). In conclusion, short supramaximal exercise can induce both hypoxaemia and increased erythropoietin levels in well-trained individuals. The decline of arterial hypoxaemia levels below 91% during exercise appears to be necessary for the exercise-induced elevation of serum erythropoietin levels. Furthermore, reflective probe pulse oximetry was found to be a valid predictor of percent arterial oxyhaemoglobin saturation during supramaximal exercise when percent estimated oxyhaemoglobin saturation > or = 86%.
本研究的目的是确定剧烈运动产生的低氧刺激是否会导致促红细胞生成素生成增加的生理反应。20名运动员在最大耗氧量的109±2.8%（平均值±标准差）下进行3分钟运动。通过反射式探头脉搏血氧饱和度仪（Nellcor N200）测量估计的氧合血红蛋白饱和度，并在8名运动员中通过一氧化碳血氧饱和度测定法与动脉氧合血红蛋白饱和度进行验证。在11名参与者中，运动后24小时使用INCSTAR Epo-Trac放射免疫分析法测量的血清促红细胞生成素浓度显著增加了28±9%，这些参与者的动脉氧合血红蛋白饱和度≤91%（P<0.05）。运动后96小时观察到铁蛋白水平降低和网织红细胞计数增加。然而，在9名未出现血氧饱和度降低的运动员和8名非运动对照组中，促红细胞生成素水平未观察到显著变化。动脉氧合血红蛋白饱和度与估计的氧合血红蛋白饱和度百分比之间显示出良好的一致性（一致性界限=-3.9至3.7%）。总之，短时间超最大运动可在训练有素的个体中诱发低氧血症和促红细胞生成素水平升高。运动期间动脉低氧血症水平降至91%以下似乎是运动诱导血清促红细胞生成素水平升高所必需的。此外，当估计的氧合血红蛋白饱和度百分比≥86%时，发现反射式探头脉搏血氧饱和度仪是超最大运动期间动脉氧合血红蛋白饱和度百分比的有效预测指标。
