Kintz P, Cirimele V, Sachs H, Jeanneau T, Ludes B
Institut de Médecine Légale, Strasbourg, France.
Forensic Sci Int. 1999 May 17;101(3):209-16. doi: 10.1016/s0379-0738(99)00024-9.
Two male bodybuilders were recently arrested by the French customs in Strasbourg (France) in possession of 2050 tablets and 251 ampoules of various anabolic steroids. It was claimed that the steroids were for personal use and not for trafficing as suggested by the police. Urine and hair specimens were collected from both suspects to clarify the claims. Nandrolone, stanozolol, testosterone and their corresponding metabolites were identified in the urine of both subjects. After decontamination, the hair was hydrolyzed by sodium hydroxide in presence of deuterated internal standards. After extraction with ethyl acetate and silylation, the drugs were identified by GC-MS in the electron impact mode. Hair from both males were positive for nandrolone (196 and 260 pg/mg), testosterone (46 and 71 pg/mg) and stanozolol (135 and 156 pg/mg), clearly indicating steroids abuse. Although not yet recognized by the International Olympic Committee, hair analysis may be a useful adjunct to conventional drug testing in urine from athletes.
两名男性健美运动员最近在法国斯特拉斯堡被法国海关逮捕，他们持有2050片及251支各种合成代谢类固醇。他们声称这些类固醇是供个人使用，而非如警方所暗示的用于贩卖。从两名嫌疑人身上采集了尿液和毛发样本以核实这些说法。在两名受试者的尿液中均鉴定出了诺龙、司坦唑醇、睾酮及其相应代谢物。毛发经净化处理后，在氘代内标存在的情况下用氢氧化钠进行水解。用乙酸乙酯萃取并进行硅烷化处理后，通过气相色谱 - 质谱联用仪在电子轰击模式下对药物进行鉴定。两名男性的毛发中诺龙（分别为196和260 pg/mg）、睾酮（分别为46和71 pg/mg）和司坦唑醇（分别为135和156 pg/mg）呈阳性，清楚地表明存在类固醇滥用情况。尽管毛发分析尚未得到国际奥委会的认可，但它可能是对运动员尿液常规药物检测的一种有用辅助手段。
