Dumestre-Toulet V, Cirimele V, Ludes B, Gromb S, Kintz P
Laboratoire BIOffice, Artigues Pres Bordeaux, France.
J Forensic Sci. 2002 Jan;47(1):211-4.
Several bodybuilders, all winners of international competitions, were arrested for trafficking of a number of doping agents including anabolic steroids, ephedrine, beta-adrenergics, human chorionic gonadotropin, antidepressants, and diuretics. In accordance with the recent French law against doping, the judge asked to test seven bodybuilders to identify doping practices. Hair and urine specimens were collected for analysis. After decontamination, a 100 mg hair strand was pulverized in a ball mill, hydrolyzed, extracted, and derivatized to be tested by GC/MS for anabolic steroids, beta-adrenergic compounds, ephedrine, and other doping agents. Urine was analyzed for anabolic steroids and metabolites, beta-adrenergic compounds, ephedrine, and human chorionic gonadotropin, in addition to a broad spectrum screening with GC/MS. The following compounds were detected in urine: ephedrine (29 and 36 ng/mL, n = 2), clenbuterol (0.2 to 0.3 ng/mL, n = 3), norandrosterone (4.7 to 100.7 ng/mL, n = 7), norethiocholanolone (0.9 to 161.8 ng/mL, n = 6), stanozolol (1 to 25.8 ng/mL, n = 4), methenolone (2.5 to 29.7 ng/mL, n = 4), testosterone (3 to 59.6 ng/mL, n = 7), epitestosterone (1 to 20.4 ng/mL, n = 7) and ratio testosterone/epitestosterone >6 for four subjects (18.5 to 59.6). The following drugs were detected in hair: ephedrine (0.67 and 10.70 ng/mg, n = 2), salbutamol (15 to 31 pg/mg, n = 3), clenbuterol (15 to 122 pg/mg, n = 6), nandrolone (1 to 7.5 pg/mg, n = 3), stanozolol (2 to 84 pg/mg, n = 4), methenolone (17 and 34 ng/ml, n = 2), testosterone enanthate (0.6 to 18.8 ng/mg, n = 5), and testosterone cypionate (3.3 to 4.8 ng/mg, n = 2). These results document the doping practice and demonstrate repetitive exposure to anabolic compounds and confirm the value of hair analysis as a complement to urinalysis in the control of doping practice.
几名国际比赛冠军健美运动员因贩卖多种兴奋剂而被捕，这些兴奋剂包括合成代谢类固醇、麻黄碱、β-肾上腺素能药物、人绒毛膜促性腺激素、抗抑郁药和利尿剂。根据法国最近出台的反兴奋剂法律，法官要求对七名健美运动员进行检测，以确定其是否使用兴奋剂。采集了头发和尿液样本进行分析。经过净化处理后，将100毫克的头发在球磨机中磨碎，进行水解、萃取和衍生化处理，然后通过气相色谱/质谱联用仪检测合成代谢类固醇、β-肾上腺素能化合物、麻黄碱和其他兴奋剂。除了用气相色谱/质谱联用仪进行广谱筛查外，还对尿液中的合成代谢类固醇及其代谢物、β-肾上腺素能化合物、麻黄碱和人绒毛膜促性腺激素进行了分析。在尿液中检测到以下化合物：麻黄碱（29和36纳克/毫升，n = 2）、克伦特罗（0.2至0.3纳克/毫升，n = 3）、去甲雄酮（4.7至100.7纳克/毫升，n = 7）、去甲硫代胆甾醇（0.9至161.8纳克/毫升，n = 6）、司坦唑醇（1至25.8纳克/毫升，n = 4）、美替诺龙（2.5至29.7纳克/毫升，n = 4）、睾酮（3至59.6纳克/毫升，n = 7）、表睾酮（1至20.4纳克/毫升，n = 7），四名受试者的睾酮/表睾酮比值>6（18.5至59.6）。在头发中检测到以下药物：麻黄碱（0.67和10.70纳克/毫克，n = 2）、沙丁胺醇（15至31皮克/毫克，n = 3）、克伦特罗（15至122皮克/毫克，n = 6）、诺龙（1至7.5皮克/毫克，n = 3）、司坦唑醇（2至84皮克/毫克，n = 4）、美替诺龙（17和34纳克/毫升，n = 2）、庚酸睾酮（0.6至18.8纳克/毫克，n = 5）和环丙孕酮睾酮（3.3至4.8纳克/毫克，n = 2）。这些结果记录了兴奋剂使用情况，证明了受试者反复接触合成代谢化合物，并证实了头发分析作为尿液分析补充手段在兴奋剂控制中的价值。
