Taniyama H, Hirayama K, Kagawa Y, Ushiki T, Kurosawa T, Furuoka H, Ono T
Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, 582-1 Bunkyodai-Midorimachi, Hokkaido 069-8501, Ebetsu, Japan.
J Comp Pathol. 1999 Aug;121(2):149-57. doi: 10.1053/jcpa.1999.0312.
The pancreatic islets were studied in seven cattle with insulin-dependent diabetes mellitus (IDDM) associated with persistent bovine viral diarrhoea virus (BVDV) infection. BVDV antigen was detected immunohistochemically in the pancreatic islet cells. There was a decrease in the size and number of islets, vacuolar degeneration of residual islet cells, and lymphocytic insulitis. The atrophied islets were composed of small uniform cells with limited amounts of cytoplasm, containing a small number of insulin- and chromogranin-positive granules. Enlarged islets consisting of islet cells with vacuolated cytoplasm were also frequently observed. Many of the vacuolated islet cells differed from the cells of normal islets in containing fewer cytoplasmic insulin- and chromogranin-positive granules. Mild lymphocytic insulitis was observed frequently in enlarged islets but rarely in atrophied islets. Immunoreactivity with BVDV antibody was found in the acinar cells of the pars exocrina in all seven cattle and in the residual cells of the islets of Langerhans of four cattle. BVDV antigen-positive cells were seen more frequently in the enlarged islets than in the atrophied islets. Some islets with lymphocytic infiltrates showed a small number of antigen-positive cells. These findings suggest that autoimmune IDDM was induced by persistent BVDV infection, resulting in gradual destruction of the islet beta cells.
对7头患有与持续性牛病毒性腹泻病毒（BVDV）感染相关的胰岛素依赖型糖尿病（IDDM）的牛的胰岛进行了研究。通过免疫组织化学方法在胰岛细胞中检测到了BVDV抗原。胰岛的大小和数量减少，残留胰岛细胞出现空泡变性，并有淋巴细胞性胰岛炎。萎缩的胰岛由细胞质有限的小而均匀的细胞组成，含有少量胰岛素和嗜铬粒蛋白阳性颗粒。还经常观察到由细胞质空泡化的胰岛细胞组成的增大的胰岛。许多空泡化的胰岛细胞与正常胰岛细胞不同，其细胞质中胰岛素和嗜铬粒蛋白阳性颗粒较少。在增大的胰岛中经常观察到轻度淋巴细胞性胰岛炎，而在萎缩的胰岛中很少见。在所有7头牛的外分泌部腺泡细胞以及4头牛的胰岛残余细胞中均发现了与BVDV抗体的免疫反应性。BVDV抗原阳性细胞在增大的胰岛中比在萎缩的胰岛中更常见。一些有淋巴细胞浸润的胰岛显示少量抗原阳性细胞。这些发现表明，持续性BVDV感染诱发了自身免疫性IDDM，导致胰岛β细胞逐渐被破坏。
