Taniyama H, Ushiki T, Tajima M, Kurosawa T, Kitamura N, Takahashi K, Matsukawa K, Itakura C
Department of Veterinary Pathology, Rakuno Gakuen University, Hokkaido, Japan.
Vet Pathol. 1995 May;32(3):221-9. doi: 10.1177/030098589503200302.
Histologic and immunohistochemical studies were carried out on four young cattle with diabetes mellitus associated with persistent bovine viral diarrhea (BVD) virus infection. Clinical findings included persistent hyperglycemia, decreased glucose tolerance, glycosuria, polydipsia, and severe emaciation. Macroscopically, multiple erosions and ulcers in the mucosa of upper and lower alimentary tracts and swollen lymph nodes were commonly observed. Erosions and ulcers in the mucosa of tongue, esophagus, and forestomach were represented histologically by necrosis of squamous epithelium with neutrophilic infiltration. In the small and large intestines, villous atrophy and suppurative cryptitis were often observed, along with diffuse infiltration of lymphocytes and macrophages and fibroplasia in the lamina propria. In the pancreas of all cattle, there was a reduction in the number of islet cells, and most of the residual islet cells had hydropic degeneration and a decreased number of secretory granules. Immunohistochemical examination confirmed that these cells were severely degranulated beta-cells. In addition, many islets containing necrotic islet cells were observed. These islet cells had increased eosinophilia and shrinkage of cytoplasm, as well as pyknotic nuclei. Inflammation of the islets with mild infiltration of lymphocytes was observed in all pancreatic lobes. In addition, bovine IgG-immunoreactive cells were identified immunohistochemically in the affected pancreatic islets. The BVD virus antigen was not identified in the cytoplasm of the islet cells by immunohistochemical study, although it was identified in the epithelial cells of the small intestine. The histologic and immunohistochemical studies demonstrated that the pancreatic lesions in these animals were similar to those caused by acute insulin-dependent diabetes mellitus (IDDM) in human beings.(ABSTRACT TRUNCATED AT 250 WORDS)
对4头患有与持续性牛病毒性腹泻（BVD）病毒感染相关的糖尿病的幼牛进行了组织学和免疫组织化学研究。临床症状包括持续性高血糖、糖耐量降低、糖尿、多饮和严重消瘦。宏观上，常见上、下消化道黏膜多处糜烂和溃疡以及淋巴结肿大。舌、食管和前胃黏膜的糜烂和溃疡在组织学上表现为鳞状上皮坏死伴嗜中性粒细胞浸润。在小肠和大肠中，常观察到绒毛萎缩和化脓性隐窝炎，同时固有层有淋巴细胞和巨噬细胞弥漫性浸润以及纤维组织增生。所有牛的胰腺中，胰岛细胞数量减少，大多数残留的胰岛细胞有细胞水肿变性且分泌颗粒数量减少。免疫组织化学检查证实这些细胞是严重脱颗粒的β细胞。此外，观察到许多含有坏死胰岛细胞的胰岛。这些胰岛细胞嗜酸性增强、细胞质收缩以及细胞核固缩。在所有胰腺叶中均观察到胰岛有轻度淋巴细胞浸润的炎症。此外，在受影响的胰岛中通过免疫组织化学鉴定出牛IgG免疫反应性细胞。免疫组织化学研究在胰岛细胞胞质中未鉴定出BVD病毒抗原，尽管在小肠上皮细胞中鉴定出了该抗原。组织学和免疫组织化学研究表明，这些动物的胰腺病变与人类急性胰岛素依赖型糖尿病（IDDM）引起的病变相似。（摘要截短至250字）
