Tajima M, Yuasa M, Kawanabe M, Taniyama H, Yamato O, Maede Y
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Zentralbl Veterinarmed B. 1999 Apr;46(3):207-15. doi: 10.1046/j.1439-0450.1999.00230.x.
In cattle, we encountered insulin-dependent diabetes mellitus (IDDM) associated with bovine viral diarrhoea virus (BVDV) infection. To estimate the correlation between IDDM and BVDV infection, the distribution of BVDV in the pancreas and islet-cell antibody (ICA) were investigated. The distribution of BVDV in the pancreas was examined by in situ hybridization using two oligonucleotide probes that recognized the gp25- and p14-coding regions of the BVDV gene. ICA was examined by indirect fluorescence antibody assay using the sera from affected cattle and pancreata from normal cattle. In the pancreata of all BVDV-infected cattle, including IDDM-complicated cattle, oligonucleotide probe hybridized portions were recognized. In short, BVDV genes were detected not only in IDDM-complicated cattle but also in uncomplicated cattle. Moreover, there was no hybridized portion in the islet cells. In BVDV-infected and IDDM-complicated cattle, ICA was frequently detected. On the other hand, ICA was not detected in BVDV-infected and IDDM uncomplicated cattle. These results suggest that IDDM associated with BVDV infection is not a direct effect of BVDV on islet cells. Therefore, as BVDV did not induce IDDM in any cases, it appears that BVDV does not induce IDDM directly, but rather may be an autoimmune disease induced by autoantibodies against islet cells.
在牛群中，我们发现胰岛素依赖型糖尿病（IDDM）与牛病毒性腹泻病毒（BVDV）感染有关。为了评估IDDM与BVDV感染之间的相关性，我们研究了BVDV在胰腺中的分布以及胰岛细胞抗体（ICA）。使用两种识别BVDV基因gp25和p14编码区的寡核苷酸探针，通过原位杂交检测BVDV在胰腺中的分布。使用患病牛的血清和正常牛的胰腺，通过间接荧光抗体试验检测ICA。在所有感染BVDV的牛的胰腺中，包括并发IDDM的牛，均识别出寡核苷酸探针杂交部分。简而言之，不仅在并发IDDM的牛中检测到BVDV基因，在未并发IDDM的牛中也检测到了。此外，在胰岛细胞中未发现杂交部分。在感染BVDV且并发IDDM的牛中，经常检测到ICA。另一方面，在感染BVDV但未并发IDDM的牛中未检测到ICA。这些结果表明，与BVDV感染相关的IDDM不是BVDV对胰岛细胞的直接作用。因此，由于在任何情况下BVDV都不会诱发IDDM，似乎BVDV不会直接诱发IDDM，而是可能是由针对胰岛细胞的自身抗体诱导的自身免疫性疾病。
