Chaslus-Dancla E, Lesage-Decauses M C, Leroy-Sétrin S, Martel J L, Coudert P, Lafont J P
Institut National de la Recherche agronomique (INRA), Centre de recherche de Tours-Nouzilly, Monnaie, France.
Vet Microbiol. 1996 Sep;52(1-2):91-102. doi: 10.1016/0378-1135(96)00065-x.
Two collections of strains of Pasteurella were studied for epidemiological purposes by ribotyping and random amplified polymorphic DNA (RAPD) assays. These strains were isolated through two different structures of animal productions: cattle and rabbit. Forty strains of P. haemolytica from cattle reared in independent breeding-herds belonged to only 3 ribotypes after digestion with HindIII and PvuII. No further discrimination of these strains was obtained by RAPD assays. All these 40 strains showed more than 90% of similarity. This result was consistent with the hypothesis of a clonal dissemination of these strains in bovine herds, possible favoured by the large use of antibiotics. Forty-one strains of P. multocida were isolated in rabbits flocks belonging to 16 breeders. Six of these were linked by commercial relationships. Twenty-eight out of the 29 strains isolated through this commercial network belonged to only three ribotypes whereas the 12 strains from independant breeders belonged to 9 ribotypes. Results of RAPD assays were in accordance with those of ribotyping and validate the use of RAPD assays for epidemiological studies of Pasteurella strains.
为了流行病学研究目的，通过核糖体分型和随机扩增多态性DNA（RAPD）分析对两株巴斯德氏菌进行了研究。这些菌株是通过两种不同的动物生产结构分离得到的：牛和兔子。从独立养殖牛群中饲养的牛身上分离出的40株溶血巴斯德氏菌在用HindIII和PvuII消化后仅属于3种核糖体分型。通过RAPD分析未对这些菌株进行进一步区分。所有这40株菌株显示出超过90%的相似性。这一结果与这些菌株在牛群中克隆传播的假设一致，可能是由于大量使用抗生素所致。在属于16个饲养者的兔群中分离出41株多杀巴斯德氏菌。其中6株通过商业关系相连。通过该商业网络分离出的29株菌株中有28株仅属于3种核糖体分型，而来自独立饲养者的12株菌株属于9种核糖体分型。RAPD分析结果与核糖体分型结果一致，并验证了RAPD分析在巴斯德氏菌菌株流行病学研究中的应用。
