Reid S D, Betting D J, Whittam T S
Institute of Molecular Evolutionary Genetics, Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
J Clin Microbiol. 1999 Aug;37(8):2719-22. doi: 10.1128/JCM.37.8.2719-2722.1999.
A multiplex PCR was designed to detect the eae gene and simultaneously identify specific alleles in pathogenic Escherichia coli. The method was tested on 87 strains representing the diarrheagenic E. coli clones. The results show that the PCR assay accurately detects eae and resolves alleles encoding the alpha, beta, and gamma intimin variants.
设计了一种多重聚合酶链反应(PCR)来检测eae基因,并同时鉴定致病性大肠杆菌中的特定等位基因。该方法在代表致泻性大肠杆菌克隆的87株菌株上进行了测试。结果表明,PCR检测能够准确检测eae基因,并分辨出编码α、β和γ紧密素变体的等位基因。