Barreau C, Conrad J, Fischer E, Lujan H D, Vernick K D
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Insect Biochem Mol Biol. 1999 Jun;29(6):515-26. doi: 10.1016/s0965-1748(99)00025-9.
Malaria transmission by the mosquito vector requires sporozoite invasion into mosquito salivary glands. Parasites probably enter the glands by specific receptor-ligand interactions with molecules on the surface of the glands. We have undertaken the characterization of salivary gland surface molecules of Aedes aegypti to identify candidate receptors for Plasmodium gallinaceum sporozoite invasion. Monoclonal antibodies (mAbs) were generated against antigen enriched for salivary gland membranes and basal lamina. A panel of 44 mAbs were generated that bound to surface molecules of mosquito tissues. Twenty-four mAbs bound exclusively to salivary glands, six bound to salivary glands and ovaries, one bound to salivary gland and midgut, and 13 bound to all tissues tested. We present data on the immunolocalization and biochemical characteristics of the antigens. Many of the salivary gland-specific mAbs bound preferentially to the median and distal lateral lobes of the salivary glands, indicating that there are anatomical region-specific biochemical differences on the gland surface. These lobes of the salivary glands are the preferential sites of malaria sporozoite invasion. Therefore, antigens specific for these regions are promising candidate receptors for sporozoite invasion. The present identification of surface molecules of mosquito salivary glands by means of monoclonal antibodies represents the first description of individual molecules on the mosquito salivary gland surface. This work lays the basis for further studies on the molecular mechanisms involved in malaria sporozoite invasion of mosquito salivary glands.
疟原虫通过蚊媒传播需要子孢子侵入蚊子的唾液腺。寄生虫可能通过与唾液腺表面分子的特定受体 - 配体相互作用进入腺体。我们已对埃及伊蚊唾液腺表面分子进行了表征，以鉴定鸡疟原虫 子孢子侵入的候选受体。针对富含唾液腺膜和基膜的抗原产生了单克隆抗体（mAb）。产生了一组44种与蚊子组织表面分子结合的单克隆抗体。24种单克隆抗体仅与唾液腺结合，6种与唾液腺和卵巢结合，1种与唾液腺和中肠结合，13种与所有测试组织结合。我们展示了这些抗原的免疫定位和生化特性数据。许多唾液腺特异性单克隆抗体优先与唾液腺的中叶和远端外侧叶结合，这表明腺体表面存在解剖区域特异性的生化差异。唾液腺的这些叶是疟原虫子孢子侵入的优先部位。因此，这些区域特异性的抗原是子孢子侵入的有前景的候选受体。目前通过单克隆抗体鉴定蚊子唾液腺表面分子代表了对蚊子唾液腺表面单个分子的首次描述。这项工作为进一步研究疟原虫子孢子侵入蚊子唾液腺所涉及的分子机制奠定了基础。
