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唾液腺对虫媒病毒传播的屏障作用的遗传基础。

The Genetic Basis for Salivary Gland Barriers to Arboviral Transmission.

作者信息

Sanchez-Vargas Irma, Olson Ken E, Black William C

机构信息

Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft. Collins, CO 80523, USA.

出版信息

Insects. 2021 Jan 15;12(1):73. doi: 10.3390/insects12010073.

DOI:10.3390/insects12010073
PMID:33467430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7830681/
Abstract

Arthropod-borne viruses (arboviruses) infect mosquito salivary glands and then escape to saliva prior to virus transmission. Arbovirus transmission from mosquitoes can be modulated by salivary gland infection barriers (SGIBs) and salivary gland escape barriers (SGEBs). We determined the influence of SGIBs and SGEBs by estimating the quantitative genetic contributions of half-sib families (Mapastepec, Mexico) infected with three dengue 2 (DENV2), two chikungunya (CHIKV), and two Zika (ZIKV) genotypes. We determined virus titer per salivary gland and saliva at seven days post-infection and virus prevalence in the half-sib population. CHIKV or ZIKV genotypes did not present SGIB, whereas DENV2 genotypes showed low rates of SGIB. However, virus titer and prevalence due to additive genetic factors in the half-sib family displayed a significant narrow-sense heritability (h) for SGIB in two of the three DENV2 genotypes and one CHIKV and one ZIKV genotype. SGEBs were detected in all seven virus strains: 60-88% of DENV2 and 48-62% of CHIKV or ZIKV genotype infections. SGEB h was significant for all CHIKV or ZIKV genotypes but not for any of the DENV2 genotypes. SGIBs and SGEBs exhibited classical gene-by-gene interaction dynamics and are influenced by genetic factors in the mosquito and the virus.

摘要

虫媒病毒（节肢动物传播病毒）感染蚊子的唾液腺，然后在病毒传播之前逃逸到唾液中。蚊子传播虫媒病毒可受到唾液腺感染屏障（SGIBs）和唾液腺逃逸屏障（SGEBs）的调节。我们通过估计感染三种登革热2型（DENV2）、两种基孔肯雅病毒（CHIKV）和两种寨卡病毒（ZIKV）基因型的半同胞家系（墨西哥马帕斯特佩克）的数量遗传贡献，来确定SGIBs和SGEBs的影响。我们在感染后7天测定了每个唾液腺和唾液中的病毒滴度以及半同胞群体中的病毒流行率。CHIKV或ZIKV基因型未表现出SGIB，而DENV2基因型显示出较低的SGIB发生率。然而，半同胞家系中由于加性遗传因素导致的病毒滴度和流行率在三种DENV2基因型中的两种以及一种CHIKV和一种ZIKV基因型中显示出SGIB的显著狭义遗传力（h）。在所有七种病毒株中均检测到SGEBs：60 - 88%的DENV2以及48 - 62%的CHIKV或ZIKV基因型感染。SGEB的h在所有CHIKV或ZIKV基因型中均显著，但在任何DENV2基因型中均不显著。SGIBs和SGEBs表现出典型的基因间相互作用动态，并且受到蚊子和病毒中的遗传因素影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/60321e733360/insects-12-00073-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/0bab58a8c7dc/insects-12-00073-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/69801c035776/insects-12-00073-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/49aa1be728a9/insects-12-00073-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/4567733f6854/insects-12-00073-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/10ac666dc167/insects-12-00073-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/5fc0586acd01/insects-12-00073-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/f732951f339c/insects-12-00073-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/18e6441f8a42/insects-12-00073-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/60321e733360/insects-12-00073-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/0bab58a8c7dc/insects-12-00073-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/69801c035776/insects-12-00073-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/49aa1be728a9/insects-12-00073-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/4567733f6854/insects-12-00073-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/10ac666dc167/insects-12-00073-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/5fc0586acd01/insects-12-00073-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/f732951f339c/insects-12-00073-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/18e6441f8a42/insects-12-00073-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e455/7830681/60321e733360/insects-12-00073-g009.jpg

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