Sionkowska A, Kamińska A
Department of Chemistry, N. Copernicus University, Toruñ, Poland.
Int J Biol Macromol. 1999 May;24(4):337-40. doi: 10.1016/s0141-8130(99)00047-1.
The thermal helix-coil transition in UV irradiated collagen solution, collagen film and pieces of rat tail tendon (RTT) were compared. Their thermal stability's were determined by differential scanning calorimeter (DSC) and by viscometric measurements. The denaturation temperatures of collagen solution, film and pieces of RTT were different. The helix-coil transition occur near 40 degrees C in collagen solution, near 112 degrees C in collagen film, and near 101 degrees C in pieces of RTT. After UV irradiation the thermal helix-coil transition of collagen samples were changed. These changes depend on the degree of hydratation.
比较了紫外线照射的胶原蛋白溶液、胶原蛋白膜和大鼠尾腱（RTT）片段中的热螺旋-线圈转变。通过差示扫描量热仪（DSC）和粘度测量来测定它们的热稳定性。胶原蛋白溶液、膜和RTT片段的变性温度不同。胶原蛋白溶液中螺旋-线圈转变发生在40℃左右，胶原蛋白膜中在112℃左右，RTT片段中在101℃左右。紫外线照射后，胶原蛋白样品的热螺旋-线圈转变发生了变化。这些变化取决于水化程度。
