Sionkowska Alina, Lewandowska Katarzyna, Adamiak Katarzyna
Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarin 7 street, 87-100 Torun, Poland.
WellU sp.z.o.o, Wielkopolska 280, 81-531 Gdynia, Poland.
Materials (Basel). 2020 Oct 8;13(19):4453. doi: 10.3390/ma13194453.
Acid soluble collagen (ASC) was extracted from fish skin. Collagen was dissolved in acetic acid at varying concentrations and its rheological properties were studied. Steady shear flow properties of collagen solutions at concentrations of 5 and 10 mg/mL were characterized using rheometry at 20 °C. Collagen solutions were irradiated with UV light (wavelength 254 nm) for up to 2 h and rheological properties were measured. All the collagen solutions showed a shear-thinning flow behavior. A constant viscosity region was observed after 1 h of UV irradiation, which showed that collagen molecules were fully denatured. A short treatment with collagen solution by UV (ultraviolet) light led to an increase in viscosity; however, the denaturation temperature of UV-irradiated collagen decreased. Depending on the time of UV treatment, collagen extracted from fish skin may undergo physical crosslinking or photodegradation. Physically crosslinked collagen may find applications in functional food, cosmetic, biomedical, and pharmaceutical industries.
从鱼皮中提取酸溶性胶原蛋白（ASC）。将胶原蛋白溶解于不同浓度的乙酸中，并研究其流变学性质。在20℃下使用流变仪对浓度为5和10mg/mL的胶原蛋白溶液的稳态剪切流动性质进行表征。用紫外线（波长254nm）照射胶原蛋白溶液长达2小时，并测量其流变学性质。所有胶原蛋白溶液均表现出剪切变稀的流动行为。紫外线照射1小时后观察到一个恒定粘度区域，这表明胶原蛋白分子已完全变性。用紫外线对胶原蛋白溶液进行短时间处理会导致粘度增加；然而，紫外线照射的胶原蛋白的变性温度降低。根据紫外线处理时间的不同，从鱼皮中提取的胶原蛋白可能会发生物理交联或光降解。物理交联的胶原蛋白可用于功能性食品、化妆品、生物医学和制药行业。
