Skrzyński S, Sionkowska A, Marciniak A
Department of Neurosurgery, Military Institute of the Health Services, Central Clinical Hospital of the Department of National Defence, Szaserów 128, 00-909 Warsaw, Poland.
J Biophys. 2009;2009:819635. doi: 10.1155/2009/819635. Epub 2010 Feb 9.
Differential scanning calorimetry (DSC) has been used to estimate the effect of disc disease on the collagen helix-coil transition and morphology for tissue extracted from patients during surgical operation. Forty discs were obtained from patients with degenerative disc disease undergoing surgery for low back pain. The patients were in the age between 20 and 70 years old. The specimens were kept wet during DSC experiment. The data allow the comparison between thermal stability of collagen tissue from healthy patients and from patients suffering from disc disease. In the paper the comparison between thermal helix-coil transition for collagen fibers from patients suffering from disc disease and collagen fibers from healthy organisms has been discussed. The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues. Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs. Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones. Disc disease induces changes in collagen structure and leads to formation of additional crosslinks between collagen fibers.
差示扫描量热法（DSC）已被用于评估椎间盘疾病对手术中从患者身上提取的组织的胶原蛋白螺旋-卷曲转变和形态的影响。从因下背痛接受手术的退行性椎间盘疾病患者身上获取了40个椎间盘。患者年龄在20至70岁之间。在DSC实验期间，标本保持湿润。这些数据使得能够比较健康患者和患有椎间盘疾病患者的胶原组织的热稳定性。在本文中，讨论了患有椎间盘疾病患者的胶原纤维与健康生物体的胶原纤维的热螺旋-卷曲转变之间的比较。加热速率对椎间盘组织中胶原蛋白变性温度的位置有影响。退变椎间盘中胶原蛋白较高的螺旋-卷曲转变温度表明胶原蛋白纤维发生了额外的分子间交联。退变男性椎间盘中胶原蛋白的变性温度值比女性的小。椎间盘疾病会引起胶原蛋白结构的变化，并导致胶原纤维之间形成额外的交联。
