不同转化等级的人尿路上皮细胞系上的癌胚抗原相关蛋白

CEA-related proteins on human urothelial cell lines of different transformation grades.

作者信息

Krop-Watorek A, Laskowska A, Salwa J, Kłopocki A G, Grunert F, Ugorski M

机构信息

Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.

出版信息

Cancer Lett. 1999 May 3;139(1):15-22. doi: 10.1016/s0304-3835(98)00363-2.

DOI:10.1016/s0304-3835(98)00363-2

PMID:10408904

Abstract

CEA family proteins from human urothelial cell lines of different transformation grades were characterized by flow cytometry and Western blotting using monoclonal antibodies: 26/3/13, D14HD11, 9A6 and 4/3/17. The following observations were made: (i) the urothelial cell lines, representing transformation grade III (TGr III, tumorigenic, invasive cells), were characterized by the presence of a component with molecular mass 110-135 kDa, most probably representing biliary glycoprotein (BGP); (ii) BGP was absent in non-tumorigenic and non-invasive TGr II urothelial cell lines; (iii) a protein band with apparent molecular mass 180 kDa, and migrating as a CEA standard was detected in only one of seven urothelial cell lines analyzed; (iv) a broad band of apparent molecular mass migrating at 65-90 kDa, probably representing NCA-50/90, was found in two tumorigenic and invasive cell lines, HCV 29T and Hu 1703He.

摘要

利用单克隆抗体（26/3/13、D14HD11、9A6和4/3/17），通过流式细胞术和蛋白质印迹法对来自不同转化等级的人尿路上皮细胞系的癌胚抗原（CEA）家族蛋白进行了表征。得出以下观察结果：（i）代表转化III级（TGr III，致瘤性、侵袭性细胞）的尿路上皮细胞系的特征是存在一种分子量为110 - 135 kDa的成分，很可能代表胆汁糖蛋白（BGP）；（ii）非致瘤性和非侵袭性的TGr II尿路上皮细胞系中不存在BGP；（iii）在所分析的七个尿路上皮细胞系中，只有一个检测到一条表观分子量为180 kDa且迁移情况与CEA标准品相同的蛋白条带；（iv）在两个致瘤性和侵袭性细胞系HCV 29T和Hu 1703He中发现了一条表观分子量在65 - 90 kDa处迁移的宽带，可能代表NCA - 50/90。