Takumi Y, Ramírez-León V, Laake P, Rinvik E, Ottersen O P
Department of Anatomy, University of Oslo, Norway.
Nat Neurosci. 1999 Jul;2(7):618-24. doi: 10.1038/10172.
Postembedding immunogold labeling was used to determine the relationship between AMPA and NMDA receptor density and size of Schaffer collateral-commissural (SCC) synapses of the adult rat. All SCC synapses expressed NMDA receptors. AMPA and NMDA receptors were colocalized in at least 75% of SCC synapses; the ratio of AMPA to NMDA receptors was a linear function of postsynaptic density (PSD) diameter, with AMPA receptor number dropping to zero at a PSD diameter of approximately 180 nm. These findings indicate that 'silent' SCC synapses are smaller than the majority of SCC synapses at which AMPA and NMDA receptors are colocalized. Thus synapse size may determine important properties of SCC synapses.
采用包埋后免疫金标记法来确定成年大鼠的谢弗侧支-连合（SCC）突触中AMPA和NMDA受体密度与大小之间的关系。所有SCC突触均表达NMDA受体。AMPA和NMDA受体在至少75%的SCC突触中共定位；AMPA与NMDA受体的比例是突触后致密部（PSD）直径的线性函数，当PSD直径约为180 nm时，AMPA受体数量降至零。这些发现表明，“沉默”的SCC突触比大多数AMPA和NMDA受体共定位的SCC突触小。因此，突触大小可能决定SCC突触的重要特性。
