Wesley C S
Laboratory of Genetics, The Rockefeller University, New York, New York 10021, USA.
Mol Cell Biol. 1999 Aug;19(8):5743-58. doi: 10.1128/MCB.19.8.5743.
The cell surface receptor Notch is required during Drosophila embryogenesis for production of epidermal precursor cells. The secreted factor Wingless is required for specifying different types of cells during differentiation of tissues from these epidermal precursor cells. The results reported here show that the full-length Notch and a form of Notch truncated in the amino terminus associate with Wingless in S2 cells and in embryos. In S2 cells, Wingless and the two different forms of Notch regulate expression of Dfrizzled 2, a receptor of Wg; hairy, a negative regulator of achaete expression; shaggy, a negative regulator of engrailed expression; and patched, a negative regulator of wingless expression. Analyses of expression of the same genes in mutant N embryos indicate that the pattern of gene regulations observed in vitro reflects regulations in vivo. These results suggest that the strong genetic interactions observed between Notch and wingless genes during development of Drosophila is at least partly due to regulation of expression of cuticle patterning genes by Wingless and the two forms of Notch.
果蝇胚胎发育过程中，细胞表面受体Notch对于表皮前体细胞的产生是必需的。分泌因子无翅（Wingless）在这些表皮前体细胞分化形成组织的过程中，对于指定不同类型的细胞是必需的。此处报道的结果表明，全长Notch以及一种氨基末端截短形式的Notch在S2细胞和胚胎中与无翅（Wingless）相关联。在S2细胞中，无翅（Wingless）和两种不同形式的Notch调节Dfrizzled 2（Wg的一种受体）、毛状基因（achaete表达的负调节因子）、蓬乱基因（engrailed表达的负调节因子）以及patched（无翅基因表达的负调节因子）的表达。对Notch突变胚胎中相同基因表达的分析表明，体外观察到的基因调控模式反映了体内的调控情况。这些结果表明，在果蝇发育过程中Notch和无翅基因之间观察到的强烈遗传相互作用，至少部分是由于无翅（Wingless）和两种形式的Notch对表皮模式形成基因表达的调控所致。
