Hill M K, Lyon K J, Lyon B R
Cooperative Research Centre for Sustainable Cotton Production, School of Biological Sciences A12, The University of Sydney, NSW, Australia.
Plant Mol Biol. 1999 May;40(2):289-96. doi: 10.1023/a:1006146419544.
Verticillium wilt is a vascular disease of cotton (Gossypium spp.) caused by the fungal pathogen Verticillium dahliae. To begin to understand the molecular mechanisms of the disease response in cotton cultivars that display superior wilt tolerance, such as Gossypium hirsutum cv. Sicala V-1, a cDNA library was constructed with mRNA isolated from root tissue of Sicala V-1, 24 h after inoculation with V. dahliae. The library was screened by a differential screening technique which was successful in identifying differences in gene expression between uninfected and V. dahliae-infected G. hirsutum root tissue. Among the differentially expressed clones, 51% represented up-regulated genes which had the potential to be involved in the defence response of G. hirsutum. The temporal expression patterns of nine suspected defence response genes were examined by northern blot analysis at several time intervals after inoculation with V. dahliae. The rapid increase in mRNA transcripts corresponding to each of these clones upon infection suggests a role for these genes in the defence response of G. hirsutum. Genes not previously associated with the defence response of the cotton plant, such as those for a 14-3-3-like protein and pathogenesis-related (PR) proteins, have been identified together with presumably novel genes, for which a definite function could not be ascribed.
黄萎病是由真菌病原体大丽轮枝菌引起的棉花维管束病害。为了初步了解表现出优异耐萎蔫性的棉花品种（如陆地棉品种Sicala V-1）的病害反应分子机制，在接种大丽轮枝菌24小时后，从Sicala V-1的根组织中分离mRNA构建了一个cDNA文库。该文库通过差异筛选技术进行筛选，该技术成功地鉴定了未感染和感染大丽轮枝菌的陆地棉根组织之间的基因表达差异。在差异表达的克隆中，51%代表上调基因，这些基因有可能参与陆地棉的防御反应。通过Northern杂交分析在接种大丽轮枝菌后的几个时间间隔检测了9个疑似防御反应基因的时间表达模式。感染后与这些克隆对应的mRNA转录本迅速增加，表明这些基因在陆地棉的防御反应中起作用。与棉花植物防御反应以前未关联的基因，如14-3-3样蛋白和病程相关（PR）蛋白的基因，已与可能的新基因一起被鉴定出来，这些新基因的明确功能尚未确定。
