Symons J D, Sun X, Flaim S F, del Balzo U
Division of Biological Sciences, Alliance Pharmaceutical Corporation, San Diego, California 92121, USA.
J Cardiovasc Pharmacol. 1999 Jul;34(1):108-15. doi: 10.1097/00005344-199907000-00018.
The efficacy of the temporary oxygen carrier perflubron emulsion (PFC) in maintaining oxygen delivery, tissue oxygenation, high-energy phosphates (HEPs), and myocardial function was investigated during low-flow ischemia. Perfusion rate, oxygen tensions, and cardiac function were measured during stabilization (5 min), controlled-flow (22 ml/min x 20 min), and low-flow (0.22 ml/min x 120 min) periods in isolated rabbit hearts. Hearts were perfused with Krebs-Henseleit (KH) solution (Control), or 10 or 20% PFC (vol/vol; n = 8 per group) 5 min before and throughout the low-flow period. Myocardial tissue was then frozen for biochemical and metabolic measurements. Myocardial oxygenation was measured at incremental flow rates by using 20% PFC (n = 4) or KH (n = 6). In PFC hearts, oxygen delivery and intramyocardial tissue Po2 were improved at all evaluated time points and flow rates, respectively (p < 0.05). In Control hearts, left ventricular end-diastolic pressure was elevated at 60, 90, and 120 min of low-flow ischemia (p < 0.05). Tissue lactate was higher (p < 0.05) and HEPs lower (p < 0.05) in Control hearts during low-flow ischemia. These results indicate that PFC treatment improves myocardial oxygenation, maintains HEPs, prevents ischemic contracture, and may increase the margin of safety during low-flow ischemia in isolated rabbit hearts.
在低流量缺血期间，研究了临时氧载体全氟溴烷乳剂（PFC）在维持氧输送、组织氧合、高能磷酸盐（HEP）和心肌功能方面的功效。在离体兔心的稳定期（5分钟）、控制流量期（22毫升/分钟×20分钟）和低流量期（0.22毫升/分钟×120分钟）测量灌注率、氧分压和心脏功能。在低流量期之前5分钟及整个低流量期，心脏用克雷布斯-亨塞尔特（KH）溶液（对照组）或10%或20%的PFC（体积/体积；每组n = 8）灌注。然后将心肌组织冷冻以进行生化和代谢测量。通过使用20%的PFC（n = 4）或KH（n = 6），在递增流速下测量心肌氧合。在PFC处理的心脏中，在所有评估的时间点和流速下，氧输送和心肌内组织氧分压分别得到改善（p < 0.05）。在对照组心脏中，低流量缺血60、90和120分钟时左心室舒张末期压力升高（p < 0.05）。在低流量缺血期间，对照组心脏中的组织乳酸水平较高（p < 0.05），HEP水平较低（p < 0.05）。这些结果表明，PFC处理可改善心肌氧合，维持HEP，预防缺血性挛缩，并可能增加离体兔心低流量缺血期间的安全边际。
