Bennett A M, Lescott T, Phillpotts R J, Mackett M, Titball R W
Defence Evaluation and Research Agency, Salisbury, Wiltshire, United Kingdom.
Viral Immunol. 1999;12(2):97-105. doi: 10.1089/vim.1999.12.97.
Recombinant vaccinia viruses that expressed the nontoxic C-domain of Clostridium perfringens alpha-toxin were constructed. The J2R (thymidine kinase [TK] gene) and B13R (serpin 2 [SPI-2] gene) loci were used as insertion sites for the clostridial DNA, and expression of the foreign protein was measured in each case. A double recombinant that encoded the alpha-toxin truncate at the B13R locus and the protective antigen of Bacillus anthracis at the J2R locus was also constructed. Although differences in expression of the alpha-toxin C-domain were recorded, all of the vaccinia recombinants protected mice against a lethal challenge with alpha-toxin demonstrating that a recombinant vaccinia virus can be used to provide protection against a toxin challenge that is known to be solely antibody mediated.
构建了表达产气荚膜梭菌α毒素无毒C结构域的重组痘苗病毒。J2R（胸苷激酶[TK]基因）和B13R（丝氨酸蛋白酶抑制剂2[SPI-2]基因）位点被用作梭菌DNA的插入位点，并分别测定了外源蛋白的表达。还构建了一种双重组体，其在B13R位点编码α毒素截短体，在J2R位点编码炭疽芽孢杆菌的保护性抗原。尽管记录了α毒素C结构域表达的差异，但所有痘苗重组体都能保护小鼠免受α毒素的致死性攻击，这表明重组痘苗病毒可用于提供针对已知仅由抗体介导的毒素攻击的保护。
