Nakamura M, Nagamine T, Ogata K, Tajima K, Aminov R I, Benno Y
Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan.
Curr Microbiol. 1999 Feb;38(2):107-12. doi: 10.1007/s002849900412.
Two small cryptic plasmids designated pONE429 and pONE430 were isolated from a rumen bacterium, Selenomonas ruminantium S20. The complete sequence of pONE429 was 2100 bp and contained one open reading frame (ORF) of 201 amino acids. The sequence of pONE430 had 1527 bp and one ORF of 171 amino acids with the similarity of replication protein (Rep protein) of pOM1, pSN2, and pIM13 isolated from Butyrivibrio fibrisolvens, Staphylococcus aureus, and Bacillus subtilis, respectively. In these plasmids, the upstream nucleotide sequence of Rep protein had the conserved nucleotides which could be double-strand origin (DSO) of rolling circle replication (RCR) mechanism. The plasmids of pONE429, pONE430, pJJMI, pJDB21, and pS23 were isolated from S. ruminantium strains and had similar regions that were located within a <450-bp nucleotide. These similar regions may be the location that was recognized by the host strain, S. ruminantium.
从瘤胃细菌反刍月形单胞菌S20中分离出两个小型隐蔽质粒，分别命名为pONE429和pONE430。pONE429的完整序列为2100 bp，包含一个由201个氨基酸组成的开放阅读框（ORF）。pONE430的序列有1527 bp和一个由171个氨基酸组成的ORF，其复制蛋白（Rep蛋白）分别与从溶纤维丁酸弧菌、金黄色葡萄球菌和枯草芽孢杆菌中分离出的pOM1、pSN2和pIM13的相似。在这些质粒中，Rep蛋白的上游核苷酸序列具有保守核苷酸，可能是滚环复制（RCR）机制的双链起点（DSO）。pONE429、pONE430、pJJMI、pJDB21和pS23质粒是从反刍月形单胞菌菌株中分离出来的，并且具有位于<450 bp核苷酸内的相似区域。这些相似区域可能是宿主菌株反刍月形单胞菌识别的位置。
