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从家鸭和家鹅中分离鉴定米氏弧菌

Isolation and identification of Vibrio metschnicovii from domestic ducks and geese.

作者信息

Hinz K H, Ryll M, Glünder G

机构信息

Clinic for Poultry, School of Veterinary Medicine Hannover, Germany.

出版信息

Zentralbl Veterinarmed B. 1999 Jun;46(5):331-9. doi: 10.1111/j.1439-0450.1999.tb01238.x.

DOI:10.1111/j.1439-0450.1999.tb01238.x
PMID:10416367
Abstract

Seven Vibrio-like field strains of German origin were isolated culturally from diseased domesticated ducks, muscovy ducks and geese, and were compared with reference strains NCTC 8443 (type strain) and NCTC 11170 of Vibrio metschnicovii using classical phenotypic and chemotaxonomic tests. Some V. cholerae strains were included in the chemotaxonomic tests for comparative purposes. On the basis of the classical phenotypic characteristics studied and the numerical analysis of the whole-cell fatty acid patterns, the Vibrio-like field strains were identified as Vibrio metschnicovii. The identification tables and the database of the computer software of two commercial micro-identification kits (API-20 NE, ID-32 E) did not identify the field strains. Of the reference strains used, only NCTC 8443 was correctly identified by the ID-32 E software.

摘要

从患病的家鸭、番鸭和鹅中通过培养分离出7株源自德国的类弧菌田间菌株,并使用经典的表型和化学分类学测试方法,将它们与溶藻弧菌的参考菌株NCTC 8443(模式菌株)和NCTC 11170进行比较。为了进行比较,在化学分类学测试中纳入了一些霍乱弧菌菌株。基于所研究的经典表型特征以及全细胞脂肪酸模式的数值分析,这些类弧菌田间菌株被鉴定为溶藻弧菌。两种商业微生物鉴定试剂盒(API-20 NE、ID-32 E)的计算机软件的鉴定表和数据库均未鉴定出这些田间菌株。在所使用的参考菌株中,只有NCTC 8443被ID-32 E软件正确鉴定。

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