Wang Yanling, Leung P C, Qian Pei-Yuan, Gu Ji-Dong
Laboratory of Environmental Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong, Hong Kong SAR, PR China.
Ecotoxicology. 2006 May;15(4):371-8. doi: 10.1007/s10646-006-0078-0. Epub 2006 May 6.
In this study, three environmental isolates of Vibrio species were isolated from water and sediment samples of Mai Po Nature Reserve, Hong Kong SAR. They were identified to be Vibrio cholerae MP-1, Vibrio aestuarianus MP-2 and Vibrio vulnificus MP-3 by both biochemical test and subsequent full-length 16S rDNA sequencing. Both disc diffusion susceptibility test and microplate dilution technique were used to investigate the antibiotic resistance profile of these three bacteria. Results from both assays were in generally good agreement. All three isolates were strongly resistant to beta-lactam family of antibiotics while susceptible to tetracycline, chloramphenicol, nalidixic acid and streptomycin. In addition, V. cholerae MP-1 was very tolerant to high concentration of vibriostatic agent O/129 to 40 microg/ml, at which the growth of V. aestuarianus MP-2 and V. vulnificus MP-2 was completely inhibited. A small naturally occurring plasmid was found in V. cholerae MP-1 while no plasmid was detected in the other two bacteria. This study suggests that Mai Po Nature Reserve harbors bacteria of unique characteristics that warrant further investigation.
在本研究中，从香港特别行政区米埔自然保护区的水样和沉积物样本中分离出三株弧菌属环境菌株。通过生化试验和随后的全长16S rDNA测序，它们被鉴定为霍乱弧菌MP-1、河口弧菌MP-2和创伤弧菌MP-3。采用纸片扩散药敏试验和微孔板稀释技术研究这三株细菌的抗生素耐药谱。两种试验结果总体吻合良好。所有三株分离株对β-内酰胺类抗生素均具有强耐药性，而对四环素、氯霉素、萘啶酸和链霉素敏感。此外，霍乱弧菌MP-1对高浓度（40μg/ml）的弧菌抑制剂O/129具有很强的耐受性，在此浓度下，河口弧菌MP-2和创伤弧菌MP-2的生长被完全抑制。在霍乱弧菌MP-1中发现了一个小的天然质粒，而在其他两种细菌中未检测到质粒。本研究表明，米埔自然保护区存在具有独特特征的细菌，值得进一步研究。
