Jurie C, Picard B, Geay Y
Laboratoire Croissance et Métabolisme des Herbivores, Institut National de la Recherche Agronomique, Theix, France.
Histochem J. 1999 Feb;31(2):117-22. doi: 10.1023/a:1003589320910.
Samples of semitendinosus muscle from 28 male cattle (18 Salers and 10 Limousins) were taken at 10 months (biopsy) and at 16 months of age (at slaughter). The animals had received the same diet and were slaughtered after the same duration of fattening. The activities of isocitrate dehydrogenase and lactate dehydrogenase were measured in the muscle samples. The five lactate dehydrogenase isoenzymes were separated by electrophoresis under non-denaturing conditions and assayed by densitometry. Fibres were identified by histochemistry by myofibrillar ATPase and succinate dehydrogenase activities as SO (slow oxidative), FOG (fast oxidative glycolytic) or FG (fast glycolytic), and by immunohistochemistry by their reaction to monoclonal antibodies specific to slow and fast myosin heavy chain reactions in I, IIC, IIA, IIAB and IIB type fibres. The isocitrate dehydrogenase activity was not modified between 10 and 16 months of age; the lactate dehydrogenase activity decreased and was correlated with an increase in the proportion of the H isozyme to the detriment of the proportion of the M form. This period was characterized by an increase in fibre size, increased expression of MHC IIa, resulting in more IIA fibres, less IIB fibres, and an increase in the percentage of type IIAB fibres, however the proportions of SO, FOG and FG, when analysed statistically, were not modified between 10 and 16 months of age.
采集了28头雄性牛（18头萨勒斯牛和10头利木赞牛）的半腱肌样本，分别在10月龄（活检）和16月龄（屠宰时）采集。这些牛的饮食相同，育肥期相同后进行屠宰。测定了肌肉样本中异柠檬酸脱氢酶和乳酸脱氢酶的活性。在非变性条件下通过电泳分离五种乳酸脱氢酶同工酶，并通过密度测定法进行分析。通过肌原纤维ATP酶和琥珀酸脱氢酶活性的组织化学方法将纤维鉴定为慢氧化型（SO）、快氧化糖酵解型（FOG）或快糖酵解型（FG），并通过免疫组织化学方法根据其对I、IIC、IIA、IIAB和IIB型纤维中慢肌球蛋白重链和快肌球蛋白重链特异性单克隆抗体的反应进行鉴定。异柠檬酸脱氢酶活性在10至16月龄之间没有变化；乳酸脱氢酶活性降低，且与H同工酶比例增加以及M型比例降低相关。这一时期的特点是纤维尺寸增加，MHC IIa表达增加，导致IIA纤维增多，IIB纤维减少，IIAB型纤维百分比增加，然而，经统计学分析，SO、FOG和FG的比例在10至16月龄之间没有变化。
