Neely A N, Clendening C E, Gardner J, Greenhalgh D G, Warden G D
Shriners Burns Hospital, Cincinnati, OH 45229, USA.
Wound Repair Regen. 1999 May-Jun;7(3):166-71. doi: 10.1046/j.1524-475x.1999.00166.x.
Keloids and hypertrophic scars are characterized by excessive deposition of collagen, which may result from insufficient protein degradation. Little is known about the levels of two gelatinases, matrix metalloproteinase-2 (72 kD type IV collagenase) and matrix metalloproteinase-9 (matrix metalloproteinase-9; 92 kD type IV collagenase) in these abnormal scars. The purpose of this study was to determine levels of these proteinases in tissue from hypertrophic scars, keloids, and donor skin. Ten hypertrophic scar samples, 9 keloid samples, and 10 donor skin samples were frozen, pulverized, homogenized, clarified by centrifugation, and analyzed for matrix metalloproteinases by quantitative zymography. Identity of matrix metalloproteinases was determined using a conditioned media reference standard, molecular weight ladders, and Western blotting. Levels of matrix metalloproteinase-9 activity were very low or undetectable in all samples. However, matrix metalloproteinase-2 activity was significantly elevated in keloids and hypertrophic scars vs. donor samples: 2.6 and 3.9-fold increases for latent matrix metalloproteinase-2, 7.8 and 6.9-fold increases for active matrix metalloproteinase-2, respectively. We conclude that little matrix metalloproteinase-9 activity (the gelatinase involved in early tissue repair) is present in keloids and hypertrophic scars, while matrix metalloproteinase-2 activity (the gelatinase involved in prolonged tissue remodeling) is present in donor skin and is significantly increased in hypertrophic scars and keloids.
瘢痕疙瘩和增生性瘢痕的特征是胶原蛋白过度沉积，这可能是由于蛋白质降解不足所致。关于这两种明胶酶，即基质金属蛋白酶-2（72 kD IV型胶原酶）和基质金属蛋白酶-9（92 kD IV型胶原酶）在这些异常瘢痕中的水平，人们了解甚少。本研究的目的是测定增生性瘢痕、瘢痕疙瘩和供体皮肤组织中这些蛋白酶的水平。将10个增生性瘢痕样本、9个瘢痕疙瘩样本和10个供体皮肤样本冷冻、粉碎、匀浆，通过离心澄清，并用定量酶谱法分析基质金属蛋白酶。使用条件培养基参考标准、分子量梯度和蛋白质印迹法确定基质金属蛋白酶的身份。在所有样本中，基质金属蛋白酶-9的活性水平非常低或无法检测到。然而，与供体样本相比，瘢痕疙瘩和增生性瘢痕中基质金属蛋白酶-2的活性显著升高：潜在基质金属蛋白酶-2分别增加2.6倍和3.9倍，活性基质金属蛋白酶-2分别增加7.8倍和6.9倍。我们得出结论，瘢痕疙瘩和增生性瘢痕中几乎不存在基质金属蛋白酶-9的活性（参与早期组织修复的明胶酶），而基质金属蛋白酶-2的活性（参与长期组织重塑的明胶酶）在供体皮肤中存在，并且在增生性瘢痕和瘢痕疙瘩中显著增加。
