Lee Weon Ju, Kim Sang Lim, Choe Yoon Seok, Jang Yong Hyun, Lee Seok-Jong, Kim Do Won
Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.
Park & Lee Skin Clinic, Daegu, Korea.
Ann Dermatol. 2015 Aug;27(4):376-82. doi: 10.5021/ad.2015.27.4.376. Epub 2015 Jul 29.
Acne is an inflammatory skin disorder caused by inflammatory biomarkers. Magnesium ascorbyl phosphate (MAP) is a stable precursor of vitamin C. It achieves a constant delivery of vitamin C into the skin and has antioxidative effects.
We performed this study to evaluate the effect of MAP on the expression of inflammatory biomarkers in cultured sebocytes.
Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay were performed for inflammatory cytokines and matrix metalloproteinases (MMPs) before and after treatment of cultured sebocytes with MAP (10(-2) M), lipopolysaccharide (LPS) (5 µg/ml) and a combination of MAP and LPS. RT-PCR and western blotting were also performed for antimicrobial peptides (AMPs) and Toll-like receptor (TLR)-4 before and after treatment of cultured sebocytes with MAP, LPS, and a combination of MAP and LPS. Quantification of lipid peroxidation was also conducted.
The increased expression of inflammatory cytokines after treatment of cultured sebocytes with LPS was decreased after treatment with MAP. MMPs, AMPs, and TLR-4 were decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS, and increased after treatment of cultured sebocytes with LPS alone. Lipid peroxidation was significantly decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS. MAP decreased the increased lipid peroxidation after treatment of cultured sebocytes with LPS.
MAP may be an effective alternative agent to improve inflammatory reactions in acne.
痤疮是一种由炎症生物标志物引起的炎症性皮肤病。磷酸镁抗坏血酸盐（MAP）是维生素C的稳定前体。它能持续向皮肤输送维生素C，并具有抗氧化作用。
我们进行本研究以评估MAP对培养的皮脂腺细胞中炎症生物标志物表达的影响。
在用MAP（10⁻² M）、脂多糖（LPS）（5 μg/ml）以及MAP与LPS的组合处理培养的皮脂腺细胞之前和之后，对炎症细胞因子和基质金属蛋白酶（MMPs）进行逆转录-聚合酶链反应（RT-PCR）和酶联免疫吸附测定。在用MAP、LPS以及MAP与LPS的组合处理培养的皮脂腺细胞之前和之后，还对抗菌肽（AMPs）和Toll样受体（TLR）-4进行RT-PCR和蛋白质印迹分析。同时进行脂质过氧化的定量分析。
用LPS处理培养的皮脂腺细胞后炎症细胞因子表达增加，而用MAP处理后这种增加有所减少。在用MAP以及MAP与LPS的组合处理培养的皮脂腺细胞后，MMPs、AMPs和TLR-4减少，而在用LPS单独处理培养的皮脂腺细胞后它们增加。在用MAP以及MAP与LPS的组合处理培养的皮脂腺细胞后，脂质过氧化显著降低。MAP降低了用LPS处理培养的皮脂腺细胞后增加的脂质过氧化。
MAP可能是改善痤疮炎症反应的一种有效替代药物。
