Renò Filippo, Grazianetti Paola, Stella Maurizio, Magliacani Gilberto, Pezzuto Carla, Cannas Mario
Human Anatomy Laboratory, Medical Sciences Department, University of Eastern Piedmont A. Avogadro, Via Solaroli 17, 28100 Novara, Italy.
Arch Dermatol. 2002 Apr;138(4):475-8. doi: 10.1001/archderm.138.4.475.
To investigate induction of matrix metalloproteinases (MMPs) during mechanical compression of hypertrophic scars. Mechanical pressure blocks hypertrophy inducted on extracellular matrix in scars by a mechanism that involves MMP-2 (gelatinase A) and MMP-9 (gelatinase B).
We assayed conditioned media obtained from normotrophic and hypertrophic scars during 24 hours of in vitro mechanical compression using gelatin zymography.
Scars from various areas of the bodies of hospitalized patients.
We obtained 3 normotrophic and 7 hypertrophic biopsy specimens from 10 patients (5 men and 5 women).
In vitro compression at a pressure of 35 mm Hg/cm(2) for 24 hours.
Vitality of scars was analyzed by means of lactic dehydrogenase test; medium samples were collected for zymographic analysis of MMP activity.
We found MMP-2 in basal (uncompressed) samples from normotrophic and hypertrophic scars. Mechanical compression induced MMP-9 release and activation (range, 86.7%-78.7%) in hypertrophic scars after 4 hours.
Production, release, and activation of MMP-9 in hypertrophic scars could be an effector mechanism responsible for hypertrophy regression induced by mechanical compression.
研究肥厚性瘢痕在机械压迫过程中基质金属蛋白酶（MMPs）的诱导情况。机械压力通过一种涉及MMP-2（明胶酶A）和MMP-9（明胶酶B）的机制来阻止瘢痕中细胞外基质的肥大诱导。
我们使用明胶酶谱法分析了在体外机械压迫24小时期间从正常瘢痕和肥厚性瘢痕获得的条件培养基。
来自住院患者身体各个部位的瘢痕。
我们从10名患者（5名男性和5名女性）身上获取了3个正常瘢痕和7个肥厚性瘢痕活检标本。
在35毫米汞柱/平方厘米的压力下进行24小时的体外压迫。
通过乳酸脱氢酶试验分析瘢痕的活力；收集培养基样本用于MMP活性的酶谱分析。
我们在正常瘢痕和肥厚性瘢痕的基础（未受压）样本中发现了MMP-2。4小时后，机械压迫诱导肥厚性瘢痕中MMP-9的释放和激活（范围为86.7%-78.7%）。
肥厚性瘢痕中MMP-9的产生、释放和激活可能是机械压迫诱导肥大消退的效应机制。
