Tanaka J, Sudo T, Ihara F, Nihira T, Yamada Y
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
Biosci Biotechnol Biochem. 1999 May;63(5):900-4. doi: 10.1271/bbb.63.900.
LipL of Pseudomonas sp. strain 109 is a unique lipase capable of catalyzing macrocyclic lactone synthesis using omega-hydroxyfatty acid esters as substrates. Several fatty acid esters were tested as inducers of LipL production. The addition of either soybean oil or a non-ionic detergent (Noigen HC) resulted in a 44 to 45-fold increase in extracellular LipL, and the presence of both resulted in a further 56-fold increase. Among the triglycerides tested, triolein was the most effective, with a 50-fold increase in LipL production. A Northern blot hybridization analysis found that the lipL transcript increased in the presence of soybean oil or Noigen HC, indicating that the production of LipL is regulated at the transcriptional level.
假单胞菌属109菌株的LipL是一种独特的脂肪酶，能够以ω-羟基脂肪酸酯为底物催化大环内酯的合成。测试了几种脂肪酸酯作为LipL产生的诱导剂。添加大豆油或非离子洗涤剂（Noigen HC）会使细胞外LipL增加44至45倍，而两者同时存在会使LipL进一步增加56倍。在所测试的甘油三酯中，三油精最有效，LipL产量增加了50倍。Northern印迹杂交分析发现，在大豆油或Noigen HC存在下，lipL转录本增加，这表明LipL的产生在转录水平上受到调控。
