Devi Rajan, Madhavan Nampoothiri Kesavan, Sukumaran Rajeev Kumar, Sindhu Raveendran, Arumugam Muthu
Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
J Basic Microbiol. 2020 Feb;60(2):112-125. doi: 10.1002/jobm.201900326. Epub 2019 Oct 30.
A newly isolated culture, Pseudomonas guariconesis, is reported for the first time for lipase production. Various process parameters affecting enzyme production were optimized through statistical design experiments. The Plackett-Burman experimental design was used for screening 10 parameters for lipase production, which was further optimized using the central composite design of response surface methodology. Maximum lipase activity of 220 U/ml was obtained after 24 h of incubation in shake-flask cultures with an inoculum concentration of 0.6% v/v, incubation temperature of 30°C, and medium pH 9.0. Castor oil (0.5% v/v) was used as the inducer for lipase production. The enzyme was found to be compatible with five different commercial detergents, indicating its potential to be used in detergent formulations. It also acted as a biocatalyst in a transesterification process. The alkaline enzyme was found to be stable in the presence of bleaching agents, metal ions, and organic solvents as well.
首次报道了一种新分离的培养物——瓜里科假单胞菌可产生脂肪酶。通过统计设计实验对影响酶产生的各种工艺参数进行了优化。采用Plackett-Burman实验设计筛选了10个脂肪酶产生参数，并进一步使用响应面法的中心复合设计进行了优化。在摇瓶培养中，接种浓度为0.6% v/v、培养温度为30°C、培养基pH值为9.0，培养24小时后，脂肪酶活性最高可达220 U/ml。蓖麻油（0.5% v/v）用作脂肪酶产生的诱导剂。发现该酶与五种不同的商用洗涤剂相容，表明其有用于洗涤剂配方的潜力。它还在酯交换过程中作为生物催化剂。发现这种碱性酶在漂白剂、金属离子和有机溶剂存在的情况下也很稳定。
