Branchet-Gumila M C, Boisnic S, Le Charpentier Y, Nonotte I, Montastier C, Breton L
Service Central d'Anatomie et Cytologie Pathologiques, Hôpital Pitié-Salpétrière, Paris, France.
Skin Pharmacol Appl Skin Physiol. 1999 Jul-Aug;12(4):211-20. doi: 10.1159/000066245.
Neurogenic inflammation of the skin observed after topical application of an irritant substance or environmental stimulation induces vascular changes and the production of inflammatory mediators. Substance P (SP) is one of the main neuropeptides which trigger an inflammatory response in the skin. So, with the aim to develop an alternative method to study neurogenic inflammation of the skin, we used an organ culture of human skin. SP was added onto epidermis or directly to culture medium in an attempt to reproduce ex vivo the effects described in vivo. Even disconnected from systemic blood circulation, in skin fragments in culture, we observed dose-dependent edema, vasodilation and extravasation of lymphocytes and mast cells through the microvascular wall. Moreover, the release of proinflammatory mediators interleukin 1alpha and tumor necrosis factor alpha was evidenced.
在局部应用刺激性物质或环境刺激后观察到的皮肤神经源性炎症会引发血管变化并产生炎症介质。P物质（SP）是引发皮肤炎症反应的主要神经肽之一。因此，为了开发一种研究皮肤神经源性炎症的替代方法，我们使用了人皮肤器官培养。将SP添加到表皮上或直接添加到培养基中，试图在体外重现体内描述的效果。即使与体循环断开连接，在培养的皮肤碎片中，我们也观察到淋巴细胞和肥大细胞通过微血管壁出现剂量依赖性水肿、血管舒张和外渗。此外，还证实了促炎介质白细胞介素1α和肿瘤坏死因子α的释放。
