人嗜T淋巴细胞病毒1型（HTLV-1）和人类免疫缺陷病毒1型（HIV-1）前病毒序列在持续感染细胞染色体中的定位。

Localization of HTLV-1 and HIV-1 proviral sequences in chromosomes of persistently infected cells.

作者信息

Glukhova L A, Zoubak S V, Rynditch A V, Miller G G, Titova I V, Vorobyeva N, Lazurkevitch Z V, Graphodatskii A S, Kushch A A, Bernardi G

机构信息

Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow.

出版信息

Chromosome Res. 1999;7(3):177-83. doi: 10.1023/a:1009243115039.

DOI:10.1023/a:1009243115039

PMID:10421377

Abstract

Integration sites for HTLV-1 and HIV-1proviruses were detected by FISH on the chromosomes of HTHIV27 cells persistently infected by HIV-1 (strain IIIB). HTLV-1 signals were found on 9 loci of chromosomes 4, 6, 9, 15 and 16. Integration sites of GC-rich HTLV-1 provirus are located in GC-rich isochores, confirming an 'isopycnic' integration, namely an integration in which the GC level of the host sequences around the integration site match the GC level of the provirus. This conclusion is not only derived from the compositional map of human chromosomes, but also from HTLV-1 hybridization on compositional fractions of human DNA. Integration of GC-poor HIV-1 provirus was found on 4 loci of chromosomes 2, 7, 17 and 19. One copy of a complete HIV-1 provirus, which is active, was integrated in H1 isochores, whereas other defective copies were located in GC-poor L isochores. These results are discussed in terms of regional integration of retroviral sequences.

摘要

通过荧光原位杂交（FISH）在被HIV-1（IIIB株）持续感染的HTHIV27细胞染色体上检测到HTLV-1和HIV-1前病毒的整合位点。在4号、6号、9号、15号和16号染色体的9个位点上发现了HTLV-1信号。富含GC的HTLV-1前病毒的整合位点位于富含GC的等密度区，证实了一种“等密度”整合，即在整合位点周围宿主序列的GC水平与前病毒的GC水平相匹配的整合。这一结论不仅来自人类染色体的组成图谱，也来自人类DNA组成部分上的HTLV-1杂交。在2号、7号、17号和19号染色体的4个位点上发现了GC含量低的HIV-1前病毒的整合。一个完整且活跃的HIV-1前病毒拷贝整合在H1等密度区，而其他缺陷拷贝位于GC含量低的L等密度区。从逆转录病毒序列的区域整合角度对这些结果进行了讨论。