人类精子中的DNA链断裂：不明原因不孕症夫妇应考虑的一个可能因素。

DNA strand breaks in human spermatozoa: a possible factor, to be considered in couples suffering from unexplained infertility.

作者信息

Høst E, Lindenberg S, Ernst E, Christensen F

机构信息

Ciconia Fertility Clinic, Frederiksberg, Denmark.

出版信息

Acta Obstet Gynecol Scand. 1999 Aug;78(7):622-5.

PMID:10422909

Abstract

BACKGROUND

The aim of this study was to assess the presence of DNA strand breaks and sperm cell morphology in men suffering from unexplained infertility, and to compare these results with normal fertile and oligozoospermic men.

METHODS

One fresh sperm sample from proven fertile sperm donors (n=20) and from infertile men with oligozoospermia, (<20 x 10(6)/ml sperm cells) (n=74), and men suffering from unexplained infertility (>20 x 10(6)/ml sperm cells) (n=39) who delivered two sperm samples with 24 hours interval, were tested for the presence of DNA strand breaks in the spermatozoa by direct immunoperoxidase detection of digoxigenin-labeled genomic DNA. Correlations to other sperm parameters, sperm cell counts, motility, activation and Krüger's strict criteria were performed.

RESULTS

DNA strand breaks in sperm cell nuclei were found significantly more often in sperm samples from men suffering from unexplained infertility compared to those from normal fertile men, and significantly more rarely compared with sperm samples from men with oligozoospermia. The percentages of normal spermatozoa (Krüger's strict criteria) were significantly lower in samples from men suffering from unexplained infertility compared to those of normal fertile men, but significantly higher compared to those of men with oligozoospermia. No difference was found between first and second day samples used for insemination, as regards DNA strand breaks, sperm cell morphology, total number of motile sperm cells, activation and motility degree.

CONCLUSION

The present data suggest that a subgroup of men suffering from unexplained infertility have DNA strand breaks in their sperm cell DNA. This group might suffer from the same malfunction as many men with oligozoospermia, however, their apoptotic activated sites in the testis are different. Delivery of sperm samples with 24 hours interval does not affect any sperm cell counts, CASA, DNA strand breaks or morphology findings in sperm samples from men suffering from unexplained infertility.

摘要

背景

本研究旨在评估不明原因不孕症男性的DNA链断裂情况和精子细胞形态，并将这些结果与正常生育男性和少精子症男性进行比较。

方法

从经证实有生育能力的精子捐献者（n = 20）、少精子症（精子细胞<20×10⁶/ml）的不育男性（n = 74）以及不明原因不孕症（精子细胞>20×10⁶/ml）的男性（n = 39）中采集一份新鲜精子样本，这些不明原因不孕症男性间隔24小时提供两份精子样本。通过直接免疫过氧化物酶检测地高辛标记的基因组DNA来检测精子中DNA链断裂的存在情况。并与其他精子参数、精子细胞计数、活力、活化情况以及克鲁格严格标准进行相关性分析。

结果

与正常生育男性的精子样本相比，不明原因不孕症男性的精子样本中精子细胞核内DNA链断裂的发现频率显著更高；与少精子症男性的精子样本相比，DNA链断裂的发现频率显著更低。与正常生育男性相比，不明原因不孕症男性样本中正常精子（克鲁格严格标准）百分比显著更低，但与少精子症男性相比则显著更高。用于授精的第一天和第二天样本在DNA链断裂、精子细胞形态、活动精子细胞总数、活化情况和活力程度方面未发现差异。