Zieren J, Castenholz E, Baumgart E, Müller J M
Klinik und Poliklinik für Chirurgie der Charité, Humboldt-Universität zu Berlin, Berlin, Germany.
J Surg Res. 1999 Aug;85(2):267-72. doi: 10.1006/jsre.1999.5608.
The purpose of this study was to investigate if the strength and quality of an abdominal wall repair with a resorbable PGA (polyglycolic acid) mesh can be improved by fibrin glue or releasates from platelets.
An abdominal wall defect in the rat was repaired using a PGA mesh in a sublay technique (CG) alone and either with additional fibrin glue (FG) or with platelet releasates (REL). Endpoints were clinical herniation pressure and hydroxyproline concentration (HP) as well as number of fibroblasts and collagen fibers at 7, 14, and 90 days after implantation.
In both experimental groups (REL and FG) higher herniation pressures, hydroxyproline contents, and number of fibroblasts/collagen fibers were found at all times of measurement compared to the CG. The PGA mesh alone showed a significant lack of stability after 14 days which can be compensated for by the investigated components. Significant differences (P < 0.05) were observed regarding the herniation pressure (REL vs CG at 7 and 14 days; FG vs CG at 14 days) and the number of collagen fibers (REL vs CG at 14 days).
These results suggest that the quality of a PGA mesh repair can be improved by application of fibrin glue or platelet releasates in the described experimental setting.
本研究的目的是调查使用可吸收聚乙醇酸（PGA）补片进行腹壁修补时，纤维蛋白胶或血小板释放物是否能改善修补的强度和质量。
采用单纯补片下层植入技术（CG），以及在植入PGA补片时额外添加纤维蛋白胶（FG）或血小板释放物（REL）来修复大鼠腹壁缺损。观察指标为植入后7天、14天和90天的临床疝形成压力、羟脯氨酸浓度（HP）以及成纤维细胞数量和胶原纤维数量。
与单纯补片组（CG）相比，在所有测量时间点，两个实验组（REL和FG）的疝形成压力、羟脯氨酸含量以及成纤维细胞/胶原纤维数量均更高。单纯PGA补片在14天后显示出明显的稳定性不足，而所研究的成分可对此进行弥补。在疝形成压力方面（7天和14天的REL组与CG组比较；14天的FG组与CG组比较）以及胶原纤维数量方面（14天的REL组与CG组比较）观察到显著差异（P < 0.05）。
这些结果表明，在上述实验环境中，应用纤维蛋白胶或血小板释放物可改善PGA补片修补的质量。
