De Luca G, Stampi S, Lezzi L, Zanetti F
Department of Medicine and Public Health, University of Bologna, Italy.
New Microbiol. 1999 Jul;22(3):203-8.
Two different decontamination systems, heat and acid, and two isolation media, GVPC and MWY agar were tested for the recovery of Legionella pneumophila from drinking water. The samples were concentrated by filtration through 0.2 micron polyamide filter and the membranes were resuspended in the original water samples. The suspension was divided into three parts: the first was placed in a 50 degrees C water bath, the second was acidified in HCl-KCl solution and the third did not undergo any treatment. The isolation was made by means of media containing charcoal, yeast extract and glycine with cycloeximide (GVPC) or vancomycin, polimixin B, anysomicin and dyes (MWY). Heating at 50 degrees C for 30 minutes was seen to be the best decontamination system above all when used with GVPC agar. Moreover, with this pretreatment higher counts were obtained both on MWY and GVPC agar. The MWY agar produced the highest isolatin percentages and the highest counts.
对两种不同的去污系统（加热和酸化）以及两种分离培养基（GVPC和MWY琼脂）进行了测试，以从饮用水中回收嗜肺军团菌。通过0.2微米聚酰胺滤膜过滤对样品进行浓缩，然后将滤膜重新悬浮于原始水样中。悬浮液分为三部分：第一部分置于50℃水浴中，第二部分用HCl-KCl溶液酸化，第三部分不进行任何处理。通过含有木炭、酵母提取物和甘氨酸以及环己酰亚胺（GVPC）或万古霉素、多粘菌素B、氨曲南和染料（MWY）的培养基进行分离。当与GVPC琼脂一起使用时，50℃加热30分钟被认为是最佳去污系统。此外，经过这种预处理后，在MWY和GVPC琼脂上均获得了更高的菌数。MWY琼脂产生的分离率最高，菌数也最高。
