Feio C L, Bauwens L, Swinne D, De Meurichy W
Laboratory of Clinical Biology, Antwerp, Belgium.
Mycoses. 1999;42(4):291-6. doi: 10.1046/j.1439-0507.1999.00450.x.
Ten Basidiobolus ranarum (= Basidiobolus haptosporus) strains, isolated from faeces of 102 different lower vertebrates (ectotherms) exhibited in Antwerp Zoo, or from their environment were studied for their temperature requirements, haemolysis and other enzyme activities in vitro. All isolates grew well at 25 and 37 degrees C. Three strains that produced undulated zygospore walls were haemolytic and positive for hyaluronidase. All the isolates produced urease, N-acetyl-beta-glucosaminidase, trypsin, lipase, lecithinase, gelatinase, collagenase and elastase, but failed to produce amylase, keratinase and beta-glucosidase. Three isolates failed to produce phosphatase. Only one strain failed to produce DNase. Aesculin was not hydrolysed. Chitinase activity was inconclusive. The results of this study illustrate the importance of exotic animals kept in temperate regions as carriers of potentially pathogenic organisms. In addition to the morphological characteristics, the identification can be based on enzymatic profiles. Enzymatic activity detection may help to explain the pathogenic mechanism of the fungus.
从安特卫普动物园展出的102种不同的低等脊椎动物（变温动物）的粪便或其环境中分离出10株蛙粪霉（= 具柄蛙粪霉）菌株，对其温度需求、溶血情况及其他体外酶活性进行了研究。所有分离株在25℃和37℃下生长良好。三株产生波浪状接合孢子壁的菌株具有溶血活性且透明质酸酶呈阳性。所有分离株均产生脲酶、N - 乙酰 - β - 葡萄糖苷酶、胰蛋白酶、脂肪酶、卵磷脂酶、明胶酶、胶原酶和弹性蛋白酶，但不产生淀粉酶、角蛋白酶和β - 葡萄糖苷酶。三株分离株不产生磷酸酶。只有一株不产生DNA酶。七叶苷未被水解。几丁质酶活性结果不明确。本研究结果表明，温带地区饲养的外来动物作为潜在致病生物载体的重要性。除形态特征外，鉴定可基于酶谱。酶活性检测可能有助于解释该真菌的致病机制。
