Bshabshe Ali Al, Joseph Martin R P, Hakami Ahmed M Al, Azraqi Tariq Al, Humayed Sulieman Al, Hamid Mohamed E
Department of Medicine, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia.
Department of Microbiology, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia.
Med Mycol. 2020 Feb 1;58(2):264-267. doi: 10.1093/mmy/myz046.
Basidiobolus species were isolated from colonic biopsy samples of patients with gastrointestinal basidiobolomycosis (GIB) in southern Saudi Arabia. Isolated fungi were initially identified using classical mycological tools and confirmed by sequence analysis of the large subunit ribosomal RNA gene. Phenotypic tests revealed zygomycete-like fungi which conform to those of Basidiobolus species. Five sequenced strains formed a monophyletic clade in the 28S ribosomal RNA gene phylogenetic tree. They shared 99.97% similarity with B. haptosporus and 99.97% with B. haptosporus var. minor, and relatively lower similarity with B. ranarum (99.925%). The study suggests a new and a serious causal agent of GIB related to Basidiobolus haptosporus. These isolates are not related to B. ranarum, which is commonly linked to this disease.
在沙特阿拉伯南部，从患有胃肠道蛙粪霉病（GIB）患者的结肠活检样本中分离出了蛙粪霉属真菌。最初使用经典真菌学工具对分离出的真菌进行鉴定，并通过对大亚基核糖体RNA基因的序列分析加以确认。表型测试显示，这些真菌类似接合菌，与蛙粪霉属真菌相符。在28S核糖体RNA基因系统发育树中，五个测序菌株形成了一个单系分支。它们与哈氏蛙粪霉的相似度为99.97%，与哈氏蛙粪霉小型变种的相似度为99.97%，而与蛙粪霉的相似度相对较低（99.925%）。该研究表明，一种与哈氏蛙粪霉相关的新型严重GIB病原体。这些分离株与通常与该病相关的蛙粪霉无关。
