Nordengrahn A, Svenda M, Moreno-Lopez J, Bergvall A, Hernandez P, McNeilly F, Allan G, Merza M
SVANOVA Biotech, National Veterinary Institute, Uppsala Science Park, Glunten, Sweden.
J Vet Diagn Invest. 1999 Jul;11(4):319-23. doi: 10.1177/104063879901100404.
A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to porcine rubulavirus (La Piedad Michoacan Virus [LPMV]) in serum samples from pigs. The test, based on a monoclonal antibody against the LPMV hemagglutinin-neuraminidase glycoprotein, had a sensitivity of 99% and a specificity of 97%. The results of this test were in agreement with those obtained by an indirect ELISA and hemagglutination inhibition, indirect immunofluorescence, and virus neutralization tests. The blocking ELISA is considered the most suitable test for routine screening for antibodies against LPMV.
开发了一种阻断酶联免疫吸附测定法（ELISA），用于检测猪血清样本中针对猪腮腺炎病毒（拉皮德米却肯病毒 [LPMV]）的抗体。该检测基于一种针对LPMV血凝素-神经氨酸酶糖蛋白的单克隆抗体，灵敏度为99%，特异性为97%。该检测结果与间接ELISA、血凝抑制、间接免疫荧光和病毒中和试验的结果一致。阻断ELISA被认为是针对LPMV抗体进行常规筛查的最合适检测方法。
