Ishigami N, Shinozuka J, Katayama K, Uetsuka K, Nakayama H, Doi K
Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
Histol Histopathol. 1999 Jul;14(3):729-33. doi: 10.14670/HH-14.729.
T-2 toxin (3 mg/kg b.w.) was orally inoculated to pregnant mice at 11 days of gestation to examine the effect of T-2 toxin on the developing embryos. At 24 hours after T-2 toxin-inoculation, moderate pyknosis or karyorrhexis was generally observed in some layers of the central nervous system, caudal sclerotomic segment, caudal region of the tongue to pharyngeal- to laryngeal-mesenchyma, trachea and facial mesenchyma. These pyknotic or karyorrhectic nuclei were strongly stained by the modified TUNEL method widely used for the in situ detection of apoptotic nuclei and also showed ultrastructural changes characteristic for apoptosis. This is the first report of mycotoxin-induced apoptosis in embryos.
在妊娠第11天给怀孕小鼠经口接种T-2毒素（3毫克/千克体重），以研究T-2毒素对发育中胚胎的影响。在接种T-2毒素24小时后，通常在中枢神经系统的某些层、尾侧硬骨节段、从舌尾侧区域到咽至喉间充质、气管和面部间充质中观察到中度核固缩或核碎裂。这些固缩或碎裂的细胞核被广泛用于原位检测凋亡细胞核的改良TUNEL法强烈染色，并且还表现出凋亡特有的超微结构变化。这是关于霉菌毒素诱导胚胎凋亡的首次报道。
